biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 43:329-336, 2000 | DOI: 10.1023/A:1026795506674

Protein Bodies in the Pine Megagametophyte in vitro Culture: Ultrastructural, Histochemical and Electrophoretic Observations

J. Høib1, R. Janisch2,*, B. Vooková1, J. Nahálková3, P. Gemeiner3, M. Hajduch1
1 Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia
2 Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic e-mail
3 Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia

The megagametophytes of the European black pine (Pinus nigra Arn.) were cultured on modified MS medium. After 10 d, protein bodies showed well-marked degradation on freeze-etched replicas and in preparations observed by scanning electron microscopy. After 20 d of cultivation, the megagametophyte cells were completely empty. Proteins secreted into the agar medium were determined by electrophoresis and 15 different proteins, in the range of 6.5 to 71 kDa, were identified.

Keywords: Pinus nigra; protein bodies degradation; proteins; SDS-PAGE
Subjects: in vitro culture, protein bodies, megagametophyte; megagametophyte, protein bodies; pine, megagametophyte; Pinus nigra; protein bodies, megagametophyte

Published: September 1, 2000  Show citation

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Høib, J., Janisch, R., Vooková, B., Nahálková, J., Gemeiner, P., & Hajduch, M. (2000). Protein Bodies in the Pine Megagametophyte in vitro Culture: Ultrastructural, Histochemical and Electrophoretic Observations. Biologia plantarum, 43(3), 329-336. doi: 10.1023/A:1026795506674
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