biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 43:353-357, 2000 | DOI: 10.1023/A:1026732618486

Burst Potential Characterisation by Capacity for Nucleotide Accumulation in Rhododendron Catawbiense Apical Buds

J. Fustec1,*, F. Beaujard2, J.D. Viémont1, M. Gendraud3
1 Faculté des Sciences, UMR SAGAH, Angers Cedex, France e-mail
2 INRA-INH, UMR SAGAH, Beaucouzé Cedex, France
3 Université Blaise Pascal, UA Bioclimatologie - PIAF, Aubière Cedex, France

Rhododendron catawbiense cv. Album propagated in vitro were transferred ex vitro and grown in a greenhouse, under long or short days. Under long days, the rhythmic growth led to an acrotonous development. In contrast, under short days, the upper buds were unable to burst, allowing basitony. In both photoperiodic conditions, the apical buds were sampled at different stages of the experiment. Growth capacities of the isolated buds were estimated by measuring their abilities to increase and diversify their non-adenylic nucleotide pool (NTP) after supplying adenosine as a precursor. Under long days, during the growth pause, the apical buds were able to increase and diversify their NTP pool. Under short days, adenosine was used to produce important quantities of ATP, while NTP pool increase became weaker. Nevertheless, during this long growth pause, apical bud tissues retained capacities to increase their NTP pool until the basal shoots developed.

Keywords: day length; growth pause; morphogenesis; non-adenylic nucleotide pool
Subjects: day length, non-adenylic nucleotide pool; growth pause, non-adenylic nucleotide pool; in vitro culture, micropropagation, nucleotide accumulation; micropropagation, tissue culture, nucleotide accumulation; morphogenesis, non-adenylic nucleotide pool; non-adenylic nucleotide pool, morphogenesis; nucleotide accumulation, tissue culture; Rhododendron catawbiense

Published: September 1, 2000  Show citation

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Fustec, J., Beaujard, F., Viémont, J.D., & Gendraud, M. (2000). Burst Potential Characterisation by Capacity for Nucleotide Accumulation in Rhododendron Catawbiense Apical Buds. Biologia plantarum, 43(3), 353-357. doi: 10.1023/A:1026732618486
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