biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:445-446, 2000 | DOI: 10.1023/A:1026739704799

Application of Esterase Isozymes for Garlic Ecotype Identification

N. Cholakova1,*
1 Institute of Genetics Acad. Doncho Kostoff, Bulgarian Academy of Sciences, Sofia, Bulgaria e-mail

Expression of esterase isozymes from cloves of bolting and non-bolting garlic clones belonging to winter and summer ecotypes were analyzed. The isozyme patterns were obtained by means of vertical block electrophoresis in polyacrylamide gels. Quantitative and qualitative differences between loci of winter and summer garlic ecotype were found. This fact substantiates application of the isoesterases for identification of garlic clones.

Keywords: Allium sativum; isoenzyme pattern; vertical block electrophoresis
Subjects: Allium sativum; ecotype identification, garlic, esterase isozymes; electrophoresis, vertical block; esterase isozymes, ecotype identification; garlic, ecotype identification; isoenzyme patterns, esterase, garlic

Published: September 1, 2000  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Cholakova, N. (2000). Application of Esterase Isozymes for Garlic Ecotype Identification. Biologia plantarum43(3), 445-446. doi: 10.1023/A:1026739704799
Download citation

References

  1. Chomátová, S., Turková, V., Klozová, E.: Protein complex and esterase isoenzyme patterns of Allium sativum L. cultivars and clones-regenerants.-Biol. Plant. 32: 321-331, 1990. Go to original source...
  2. Kusnetzov, A.V.: Chesnok Kul'turnyï. [The cultivated garlic].-Sel'skokhoz. Izd., Moskva 1954. [In Russ.]
  3. Maas, H.I., Klaas, M.: Intraspecific differentiation of garlic (Allium sativum L.) by isozyme and RAPD markers.-Theor. appl. Genet. 91: 89-97, 1995. Go to original source...
  4. Markova, M., Vodenicharova, M., Stoilova, Ts., Cholakova, N.: Application of seed esterase isoenzymes in testing hybridity of tomato.-Biol. Plant. 41: 265-269, 1998. Go to original source...
  5. Peacock, A., Bunting, S., Queen, K.: Serum protein electrophoresis in acrylamide gel: patterns from normal human subjects.-Science 147: 1451-1453, 1965. Go to original source...
  6. Pooler, M.R., Simon, P.W.: Characterization and classification of isozyme and morphological variation in a diverse collection of garlic clones.-Euphytica 68: 121-130, 1993. Go to original source...
  7. Shaw, C., Prassad, R.: Starch gel electrophoresis of enzyme-a compilation of recipes.-Biochem. Genet. 4: 297-320, 1970. Go to original source...