biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:447-450, 2000 | DOI: 10.1023/A:1026743822546

Micropropagation in Peanut (Arachis hypogaea L.)

T. Radhakrishnan1,*, T.G.K. Murthy1, K. Chandran1, A. Bandyopadhyay1
1 National Research Centre for Groundnut, Junagadh, India e-mail

Multiple shoots in Arachis hypogaea L. could be induced from the de-embryonated cotyledons (DC), embryo-axes (EA) and mature whole seeds (MWS) in MS medium supplemented with different levels of benzylaminopurine (BAP). DC was the most suitable explant with 57.9 % induction and more than 40 shoots per explant in 31.6 % of cases. Though EA and MWS had high percent induction at or above 30 mg dm-3 BAP, only 10 - 14 shoots per explant were observed. In DC, multiple shoots were confined to the proximal end and in EA they originated from the axillary bud region. Histological studies on DC confirmed the origin of shoots from the region of attachment with the embryo. Shoots could be rooted in MS medium containing 2 g dm-3 charcoal and 200 mg dm-3 casein hydrolysate. Sixty percent of the rooted plantlets could be established in the field.

Keywords: embryo-axes; de-embryonated cotyledons; multiple shoots; histology
Subjects: Arachis hypogaea; cotyledons, de-embryonated, in vitro culture; in vitro culture, multiple shoots; multiple shoots, in vitro culture; peanut, micropropagation

Published: September 1, 2000  Show citation

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Radhakrishnan, T., Murthy, T.G.K., Chandran, K., & Bandyopadhyay, A. (2000). Micropropagation in Peanut (Arachis hypogaea L.). Biologia plantarum, 43(3), 447-450. doi: 10.1023/A:1026743822546
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