biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 43:463-466, 2000 | DOI: 10.1023/A:1026756124363

Activity of Some Aminopeptidases in Immobilized Cells of Daucus carota

L. Hegedus1, J. Vojta¹¹ák2, L. Bilisics2, N.V. Borovkov2, P. Siekel3,*
1 EBA, Ltd., Bratislava, Slovak Republic
2 Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic
3 Food Research Institute, Bratislava, Slovak Republic e-mail

Carrot cell suspension culture prior to immobilization by glutaraldehyde was permeabilised by Tween 80. The values of pH optimum for L-alanine aminopeptidase, L-proline iminopeptidase, and L-arginine aminopeptidase were 8.2, 7.4, and 7.9, respectively. The immobilized cells showed significantly lower aminopeptidase activity when compared to untreated cells. Alginate hydrogel was successfully used for immobilization of carrot cells retaining the activity of some aminopeptidases.

Keywords: alginate; carrot; cell permeabilization; glutaraldehyde

Published: September 1, 2000  Show citation

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Hegedus, L., Vojta¹¹ák, J., Bilisics, L., Borovkov, N.V., & Siekel, P. (2000). Activity of Some Aminopeptidases in Immobilized Cells of Daucus carota. Biologia plantarum, 43(3), 463-466. doi: 10.1023/A:1026756124363
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