biologia plantarum

Biologia plantarum 43:477-480, 2000 | DOI: 10.1023/A:1026716527089

In Vitro Selection for Salt Tolerance in Rice

D. Shankhdhar¹, S.C. Shankhdhar¹, S.C. Mani², R.C. Pant¹

¹ Department of Plant Physiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar -, U.P., India e-mail

² Department of Genetics and Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar -, U.P., India

In six cultivars of rice (Oryza sativa L.), Pusa Basmati 1, Basmati 370, Type III, Pant Dhan 4, CSR 10 and Pokkali, embryogenic callus growth, plant regeneration, and proline and total protein contents were studied under salt stress (on agar solidified media containing 0, 0.5, 1.0, 1.5 and 2.0 % NaCl). Four weeks after inoculation the callus fresh mass decreased with increasing salt concentration in all the six cultivars. The regeneration frequency in salt stressed callus was also lower as compared to control. 15 d and 30 d after inoculation proline content increased several fold whereas total protein content decreased markedly with increase in salt concentration.

Keywords: callus growth; Oryza sativa; proline; protein; salt stress

Subjects: callus growth, in vitro culture, salt tolerance; in vitro culture, callus growth, salt tolerance; in vitro culture, salt tolerance, proline; Oryza sativa; proline, in vitro culture, salt tolerance; proline, salt stress; rice, in vitro culture, salt tolerance

Published: September 1, 2000  Show citation