biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:517-522, 2000 | DOI: 10.1023/A:1002846105299

Temporal Changes in the RNA Distribution between Polysomes and Postpolysomal Ribonucleoprotein Particles in Tobacco Male Gametophyte

D. Honys1,*, V. Čapková1
1 Laboratory of Pollen Biology, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic e-mail

Growth of tobacco (Nicotiana tabacum L.) pollen tube is controlled by post-transcriptionally regulated protein synthesis. Stored mRNA was found to be present in the form of messenger ribonucleoprotein particles (mRNPs) in both maturing and germinating pollen. During pollen dehydration in the anthers content of mRNPs strongly increased during dissociation of polysomes suggesting a transfer of mRNA liberated from polysomal structures into mRNP particles. Pollen germination and initial pollen tube growth characterized by rapid reassociation of ribosomes was accompanied by decrease of mRNPs indicating an involvement of mRNA of these particles in the formation of polysomes. Distribution of the particular mRNAs during the pollen activation preceeding tube growth suggests that other mechanisms along with pollen rehydration are engaged in this process and that the continuous exploitation of stored mRNPs during pollen tube growth is precisely regulated.

Keywords: in vitro translation; Nicotiana tabacum L.; pollen; polysomes; stored mRNA; translational regulation
Subjects: in vitro translation, pollen germination; mRNA stored, pollen germination; Nicotiana tabacum; pollen germination, mRNA; polysomes, RNA distribution; postpolysomal ribonucleoprotein particles in male gametophyte; RNA distribution, temporal changes; tobacco male gametophyte; translational regulation, pollen germination

Published: December 1, 2000  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Honys, D., & Čapková, V. (2000). Temporal Changes in the RNA Distribution between Polysomes and Postpolysomal Ribonucleoprotein Particles in Tobacco Male Gametophyte. Biologia plantarum, 43(4), 517-522. doi: 10.1023/A:1002846105299
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Beltrán-Peña, E., Ortíz-López, A., Sánchez de Jiménez, E.: Synthesis of ribosomal proteins from stored mRNAs early in seed germination.-Plant mol. Biol. 28: 327-336, 1995. Go to original source...
    2. Chomczynski, P., Sacchi, N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction.-Anal. Biochem. 162: 156-159, 1987. Go to original source...
    3. Čapková, V., Hrabětová, E., Tupý, J.: Protein changes in tobacco pollen culture: a newly synthesized protein related to pollen tube growth.-J. Plant Physiol. 130: 307-314, 1987. Go to original source...
    4. Čapková, V., Hrabětová, E., Tupý, J., Říhová, L.: Amino acid uptake and protein synthesis in cultured tobacco pollen.-Biochem. Physiol. Pflanzen 178: 511-520, 1983.
    5. Davidson, E.H.: Gene Activity in Early Development.-Academic Press, New York 1976.
    6. De Vries, S., Hoge, H., Bisseling, T.: Isolation of total and polysomal RNA from plant tissues.-In: Plant Molecular Biology Manual B6. Pp. 1-13. Kluwer Academic Publishers, Dordrecht 1988. Go to original source...
    7. Goldberg, R.B.: Plants: novel developmental processes.-Science 240: 1460-1467, 1988. Go to original source...
    8. Heslop-Harrison, J., Heslop-Harrison, Y.: Germination of monocolpate angiosperm pollen: affect of inhibitory factors and the calcium-channel blocker, nifedipine.-Ann. Bot. 69: 395-403, 1992. Go to original source...
    9. Katsu, Y., Yamashita, M., Nagahama, Y.: Isolation and characterization of goldfish Y box protein, a germ-cell-specific RNA-binding protein.-Eur. J. Biochem. 249: 854-861, 1997.
    10. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    11. Linskens, H.F., Schrauwen, J.A.M., Konings, R.N.H.: Cell-free protein synthesis with polysomes from germinating Petunia pollen grains.-Planta 90: 153-162, 1970. Go to original source...
    12. Mascarenhas, J.P., Bell, E.: Protein synthesis during germination of pollen: Studies of polyribosome formation.-Biochim. biophys. Acta 179: 199-203, 1969. Go to original source...
    13. Mascarenhas, J.P.: Molecular mechanism of pollen tube growth and differentiation.-Plant Cell 5: 1303-1314, 1993. Go to original source...
    14. Minich, W.B., Korneyeva, N.L., Ovchinnikov, L.P.: Translational active mRNPs from rabbit reticulocytes are qualitetively different from free mRNA in their translatability in cell-free system.-FEBS Lett. 257: 257-259, 1989. Go to original source...
    15. Pierandrei-Amaldi, P., Amaldi, F.: Aspects of regulation of ribosomal protein synthesis in Xenopus laevis.-Genetica 94: 181-193, 1994. Go to original source...
    16. Pramanik, S.K., Krochko, J.E., Bewley, J.D.: Distribution of cytosolic mRNAs between polysomal and ribonucleoprotein complex fractions in alfalfa embryos.-Plant Physiol. 99: 1590-1596, 1992. Go to original source...
    17. Rincón-Guzmán, A., Beltrán-Peña, E., Ortíz-López, A., Sánchez de Jiménez, E.: Ribonucleoprotein particles of quiescent maize embryonic axes.-Plant mol. Biol. 38: 357-364, 1998. Go to original source...
    18. Schrauwen, J.A.M., De Groot, P.F.M., Van Herpen, M.M.A., Van der Lee, T., Reynen, W.H., Weterings, K.A.P., Wullems, G.J.: Stage-related expression of mRNAs during pollen development in lily and tobacco.-Planta 182: 298-304, 1990. Go to original source...
    19. Silverstein, E.: Subribosomal ribonucleoprotein particles of developing wheat embryo.-Biochemistry 12: 951-958, 1973. Go to original source...
    20. Spirin, A.S., Nemer, M.: Messenger RNA in early sea urchin embryos: cytoplasmic particles.-Science 150: 214, 1965. Go to original source...
    21. Tupý, J.: Alterations in polyadenylated RNA during pollen maturation and germination.-Biol. Plant. 24: 331-340, 1982. Go to original source...
    22. Tupý, J., Hrabětová, E., Balatková, V.: Evidence for ribosomal RNA synthesis in pollen tubes in culture.-Biol. Plant. 19: 226-230, 1977. Go to original source...
    23. Tupý, J., Říhová, L.: Changes and growth effect of pH in pollen culture.-J. Plant Physiol. 115: 1-10, 1984. Go to original source...
    24. Tupý, J., Süss, J., Hrabětová, E., ŘíhováL.: Developmental changes in gene expression during pollen differentiation and maturation in Nicotiana tabacum L.-Biol. Plant. 25: 231-237, 1983. Go to original source...
    25. Tupý, J., Süss, J., Říhová, L.: RNA synthesis and ribosome status in pollen tube growth of Nicotiana tabacum L.-J. Plant Physiol. 123: 467-476, 1986. Go to original source...
    26. Wood, A.J., Oliver, M.J.: Translational control in plant stress: the formation of messenger ribonucleoprotein particles (mRNPs) in response to dessication of Tortula ruralis gametophytes.-Plant J. 18: 359-370, 1999. Go to original source...

    Return to the content