biologia plantarum

Biologia plantarum 43:577-582, 2000 | DOI: 10.1023/A:1002850522080

Growth and Protein Content in Colletotrichum circinans, Fusarium solani and Rhizoctonia solani in Liquid Culture

M. Juarez¹, D. Juarez¹, V.M. Russo^2,*

¹ Biology Department, East Central University, Ada, USA

² South Central Agricultural Research Laboratory, USDA, ARS, Lane, USA e-mail

The phytopathogenic fungi Colletotrichum circinans, Fusarium solani, and Rhizoctonia solani were incubated in aerated (0, 0.5, 1 dm³ min^-1) potato dextrose broth (PDB) or Czapek-Dox broth (CDB), under 0-, 12- or 24-h photoperiods. Greater dry mass was produced in PDB. Higher air flows improved dry mass of F. solani and R. solani. The 24-h photoperiod improved F. solani dry mass. Except for F. solani, which was not affected, incubation in PDB increased protein content. The no air treatment increased protein content in F. solani, 0.5 dm³ min^-1 produced the highest protein content in R. solani, but air flow-rate did not affect C. circinans. Incubation in the dark produced the lowest protein content in C. circinans, the highest under the 24-h photoperiod for R. solani, and photoperiod did not affect protein content in F. solani. Protein content in R. solani, incubated in CDB, was lowest at the 0 dm³ min^-1 air flow at all photoperiods. Molecular masses of most proteins were under 30 kDa, and numbers of bands in SDS-PAGE gels varied with air flow. In CDB, especially under 12- or 24-h photoperiods, proteins in F. solani were between 1.6 and 310 kDa. For R. solani in PDB, at 0.5 dm³ min^-1 air flow and 12-h light, proteins were between 6 and 81 kDa.

Keywords: air flow; fluorescent light; media

Subjects: Colletotrichum circinans; fluorescent light, fungal growth, proteins; fungi, photoperiod, growth and protein content in different liquid media; Fusarium solani; Rhizoctonia solani

Published: December 1, 2000  Show citation