biologia plantarum

Biologia plantarum 43:591-594, 2000 | DOI: 10.1023/A:1002815015236

Effect of Combined Salt and Heat Treatments on Germination and Heat-Shock Protein Synthesis in Lentil Seeds

A. Dell'Aquila¹

¹ Germplasm Institute, CNR, Bari, Italy e-mail

The germination of lentil seeds was gradually reduced when seeds were exposed to temperature of 30 or 40 °C, either alone or combined with 0.1, 0.2 or 0.3 M NaCl or 34.1 % (m/v) PEG 8000, during 6 -12 h imbibition. [³⁵S]-methionine incorporation in 12 h imbibed lentil axes also decreased with increasing NaCl concentration at 20 and 40 °C, whereas at 30 °C only 0.3 M NaCl treatment partially inhibited protein synthesis. An analysis of newly synthesized proteins by 1-D SDS PAGE, showed that the expression of most polypeptides decreased following increasing stress. Among these, low molecular mass heat-shock proteins declining, higher in 40 °C treated axes than those treated at 30 °C, supports the hypothesis that at this temperature maximal level of expression of these proteins was achieved.

Keywords: heat stress; Lens culinaris; salt stress

Subjects: heat and salt effect, combined, germination; heat shock protein, synthesis, germination; Lens culinaris; lentil, heat and salt effect, combined; salt and heat effect, combined, germination

Published: December 1, 2000  Show citation