biologia plantarum

Biologia plantarum 43:633-636, 2000 | DOI: 10.1023/A:1002820620688

Leaf Structure of Tobacco In Vitro Grown Plantlets as Affected by Saccharose and Irradiance

B. Radochová¹, A. Vičánková¹, J. Kutík¹, I. Tichá¹

¹ Faculty of Science, Department of Plant Physiology, Charles University Prague, Praha 2, Czech Republic

Tobacco plantlets were cultured in vitro under high (200 µmol m^-2 s^-1) or low (60 µmol m^-2 s^-1) irradiance with or without saccharose in the medium. Light microscopy and image analysis were used to evaluate the effect of these culture conditions on leaf anatomy. Addition of saccharose resulted in thicker leaves (all leaf layers) and larger mesophyll cells under both growth irradiances. Various irradiance affected leaf anatomy differently when plantlets had been cultivated in presence or absence of saccharose in the medium. While under high irradiance in presence of saccharose leaf thickness and number of chloroplasts per cell section were increased, plantlets grown under high irradiance in absence of saccharose had thinner leaves and less chloroplasts per cell section. The changes were more pronounced in palisade parenchyma layer.

Keywords: epidermis; leaf anatomy; Nicotiana tabacum; spongy parenchyma layer

Subjects: epidermis, in vitro culture, sucrose, irradiance; in vitro culture, epidermis, sucrose, irradiance; irradiance, in vitro culture, sucrose; leaf anatomy, in vitro culture, sucrose, irradiance; Nicotiana tabacum; spongy parenchyma, in vitro culture, sucrose, irradiance; tobacco, in vitro culture, sucrose, irradiance

Published: December 1, 2000  Show citation