biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:7-12, 2001 | DOI: 10.1023/A:1017901817063

Efficient In Vitro Micropropagation and Regeneration of Humulus Lupulus on Low Sugar, Starch-Gelrite Media

I. Smýkalová1, M. Ortová1, H. Lipavská2, J. Patzak3
1 Laboratory of Tissue Culture, Faculty of Agronomy, Czech University of Agriculture, Prague 6 - Suchdol, Czech Republic
2 Department of Plant Physiology, Faculty of Science, Charles University, Prague 2, Czech Republic
3 Hop Research Institute Co., Ltd., ®atec, Czech Republic

Several Czech and foreign hop mericlones were tested in vitro for efficiency of green callus formation and plant regeneration from internodal or nodal explants. Modified MS media gelled either with agar, starch or a mixture of potato starch and Gelrite, supplemented with different concentrations of either glucose or maltose, were investigated. Two mericlones of Czech hop (Osvald 72 no. 5216 and Sládek no. 6908) were studied in more details because of their different regeneration capacities. The HPLC analysis of medium sugar concentrations after the explant cultivation has revealed slow uptake of sugar from the medium. Presence of glucose at concentration of 45 g dm-3 in agar medium resulted in a decreased number of nodes compared to the control with 30 g dm-3 of glucose. The use of a mixture of potato starch plus Gelrite instead of routinely used agar and decreasing the medium glucose concentration to 15 g dm-3 proved to be most efficient for multiplication rate. The use of this medium results in lower cost of micropropagation of healthy hop cultures without exhibition of vitrification.

Keywords: gelling agents; glucose; hop; maltose; micropropagation
Subjects: cytokinins; gelling agents, in vitro culture; glucose, micropropagation on low sugar media; hop, in vitro micropropagation; Humulus lupulus; in vitro culture, sugars as C source, low sugar media; maltose, in vitro culture; photoautotrophy, in vitro culture; photomixotrophy, in vitro culture

Published: March 1, 2001  Show citation

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Smýkalová, I., Ortová, M., Lipavská, H., & Patzak, J. (2001). Efficient In Vitro Micropropagation and Regeneration of Humulus Lupulus on Low Sugar, Starch-Gelrite Media. Biologia plantarum, 44(1), 7-12. doi: 10.1023/A:1017901817063
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