biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:13-18, 2001 | DOI: 10.1023/A:1017953801133

Role of Amino Acids in Evolution of Ethylene and Methane, and Development of Microshoots in Cajanus Cajan

G.V. Sudarsana1, R. Chandra1, R. Polisetty1
1 Plant Tissue Culture Laboratory, Division of Plant Physiology, I.A.R.I., New Delhi-, India

In pigeonpea (Cajanus cajan L.) shoot buds were induced when cotyledonary node explants were supplemented with benzylaminopurine (BAP; 2 mg dm-3). When 0.1 mg dm-3 BAP and 0.01 mg dm-3 naphtahalene acetic acid were supplemented to the medium, the 34 - 35 % of induced buds developed to microshoots. By supplementing amino acids like proline, glutamine, asparagine and L-cysteine, shoot bud development to microshoots was enhanced at least by two fold. Amongst the amino acids proline gave maximum number of microshoots per explant. With increase in concentration of amino acids, fresh mass increased but microshoot number decreased. Also methane evolution was increased by addition of amino acids, and also in medium containing more of its nitrogen in the form of ammonia. Increased evolution of methane was accompanied by reduction in evolution of ethylene, and enhancement of efficiency of microshoot development.

Keywords: asparagine; glutamine; L-cysteine; pigeonpea; proline
Subjects: amino acids, microshoots development in vitro; asparagine, microshoot development; auxins; Cajanus cajan; cysteine, microshoot development; cytokinins; ethylene evolution in vitro, microshoot development; glutamine, microshoot development; in vitro culture, micropropagation, regeneration; methane evolution in vitro , microshoot development; pigeonpea; proline, microshoot development

Published: March 1, 2001  Show citation

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Sudarsana, G.V., Chandra, R., & Polisetty, R. (2001). Role of Amino Acids in Evolution of Ethylene and Methane, and Development of Microshoots in Cajanus Cajan. Biologia plantarum, 44(1), 13-18. doi: 10.1023/A:1017953801133
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