biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:33-39, 2001 | DOI: 10.1023/A:1017910018880

Protein Changes Associated with Adventitious Root Formation in Hypocotyls of Pinus Radiata

M. Li1, D.W.M. Leung1
1 Department of Plant & Microbial Sciences, University of Canterbury, Christchurch, New Zealand

The changes in soluble proteins associated with adventitious root formation in hypocotyls of radiata pine (Pinus radiata D. Don) were studied using one- and two-dimensional polyacrylamide gel electrophoresis. Protein content decreased during the first day after root excision, and kept decreasing till the end of the time course under non-rooting conditions, i.e., on medium without growth regulators, with indole-3-butyric acid (IBA) + kinetin, or with kinetic alone. During adventitious root initiation in response to IBA, however, the protein content began to increase from day 1 to its maximum at day 7, coinciding with the early stage of root initiation. A comparative analysis of protein changes by two-dimensional polyacrylamide gel electrophoresis showed 16 proteins that were probably associated with root initiation and development.

Keywords: indole-3-butyric acid; kinetic; pine; protein patterns
Subjects: adventitious root formation, proteins; auxins; cytokinins; hypocotyl, adventitious root formation; kinetin, adventitious root formation, proteins; pine; Pinus radiata

Published: March 1, 2001  Show citation

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Li, M., & Leung, D.W.M. (2001). Protein Changes Associated with Adventitious Root Formation in Hypocotyls of Pinus Radiata. Biologia plantarum, 44(1), 33-39. doi: 10.1023/A:1017910018880
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