biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:131-135, 2001 | DOI: 10.1023/A:1017999127966

Effect of Nitrate and Aminoethoxyvinylglycine on Cicer Arietinum L. Nodules

A. Mann1, A.S. Nandwal2, B.S. Kundu2, S. Sheokand1, B. Kumar1, D. Datta1, A. Sheoran2
1 Department of Botany, CCS Haryana Agricultural University, Hisar, Haryana, India
2 Department of Microbiology, CCS Haryana Agricultural University, Hisar, Haryana, India

The chickpea (Cicer arietinum L.) cv. HC-1 was raised in earthen pots filled with dune sand in screenhouse. At vegetative stage, i.e. 40 - 45 d after sowing, 10, 20 and 40 mM NO3- was applied through rooting medium. After 24 h of NO3- treatments an ethylene biosynthesis inhibitor, aminoethoxyvinylglycine (AVG) in concentration 5 μM was given. A conspicuous increase in (5 - 9 fold) ethylene evolution in nodules was noticed after NO3- treatments. This rise was parallel to the increase in 1-aminocyclopropane-1-carboxylic acid (ACC) content and ACC oxidase activity. On the contrary, a sharp decline in ACC content, ACC oxidase activity and ethylene evolution was observed when AVG was given. A decrease of in acetylene reduction assay (ARA) with NO3- treatments was associated with decline in cytosolic pH (from 6.12 to 5.45), leghemoglobin (Lb) content, accumulation of H2O2 and with the loss of membrane integrity. The lipid peroxidation, followed as MDA production and electrolyte leakage increased with NO3- treatments, however, the level of MDA was brought down in AVG-treated nodules. Results confirm that ethylene might be involved in mechanism by which the functioning of nodules is adversely affected by NO3-.

Keywords: chickpea; ethylene; 1-aminocyclopropane-1-carboxylic acid; ACC oxidase; N2-fixation; membrane integrity; cytosolic pH
Subjects: a1-aminocyclopropane-1-carboxylic acid (ACC) oxidase; chickpea; Cicer arietinum; cytosolic pH, nitrate; dinitrogen fixation, nodules; ethylene biosynthesis, nodules; membrane integrity, nodules; N2 fixation, nodules; nodules, nitrogen fixation

Published: March 1, 2001  Show citation

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Mann, A., Nandwal, A.S., Kundu, B.S., Sheokand, S., Kumar, B., Datta, D., & Sheoran, A. (2001). Effect of Nitrate and Aminoethoxyvinylglycine on Cicer Arietinum L. Nodules. Biologia plantarum, 44(1), 131-135. doi: 10.1023/A:1017999127966
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    References

    1. Abeles, F.B., Morgan, P.W., Saltveit, M.E., Jr.: Ethylene in Plant Biology. 2 Ed.-Academic Press, New York 1992.
    2. Caba, J.M., Recalde, L., Ligero, F.: Nitrate induced ethylene biosynthesis and the control of nodulation in alfalfa.-Plant Cell Environ. 21: 87-93, 1998. Go to original source...
    3. Chamber-Perez, M.A., Camacho, M.M., Soriano, N.J.J.: Nitrate-reductase activities of Bradyrhizobium spp. in tropical legumes: Effects of nitrate on O2 diffusion in nodules and carbon costs of N2-fixation.-J. Plant Physiol. 150: 92-96, 1997. Go to original source...
    4. Corey, K., Barker, A.: Ethylene evolution and polyamine accumulation by tomato subjected to interactive stresses of ammonium toxicity and potassium deficiency.-J. amer. Soc. hort. Sci. 114: 651-655, 1989. Go to original source...
    5. De Lorenzo, C.A., Fernandez-Pascual, M.M., De Felipe, M.R.: Protective enzymes against active oxygen species during nitrate induced senescence of Lupinus albus nodules.-J. Plant Physiol. 144: 633-640, 1994. Go to original source...
    6. Escuredo, P.R., Minchin, F.R., Gogorcena, Y., Itube-Ormaetxe, L., Klucas, R.V., Becana., M.: Involvement of activated oxygen in nitrate-induced senescence of pea root nodules.-Plant Physiol. 110: 1187-1195, 1996. Go to original source...
    7. Fearn, J.C., La Rue, T.A.: Ethylene inhibitors restore nodulation of sym 5 mutants of Pisum sativum cv. "Sparkle".-Plant Physiol. 96: 239-244, 1991. Go to original source...
    8. Hardy, R.W.F., Holsten, R.D., Jackson, E.K., Burns, R.C.: The acetylene ethylene essay for N2-fixation: Laboratory and field evaluation.-Plant Physiol. 43: 1185-1207, 1968. Go to original source...
    9. Hartree, E.F.: Haematin compounds.-In: Peach, K., Tracey, M.V. (ed.): Modern Methods of Plant Analysis. Pp. 197-245. Springer-Verlag, Berlin 1955. Go to original source...
    10. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189-198, 1968. Go to original source...
    11. Kacperska, A., Kubacka-Zebalska, M.: Wound ethylene synthesis in the stress-affected cells.-In: Peach, J.C., Latche, A., Balague, C. (ed.): Cellular and Molecular Aspects of the Plant Hormone Ethylene. Pp. 212-216. Kluwer Academic Publishers, Dordrecht 1993. Go to original source...
    12. Klucas, R.V., Arp, D.: Physiological and biochemical studies on senescing tap root nodules of soybeans.-Can. J. Microbiol. 23: 1426-1432, 1977. Go to original source...
    13. Ligero, F., Caba, J.M., Lluch, C., Olivares, J.: Nitrate inhibition of nodulation can be overcome by the ethylene inhibitor aminoethoxyvinylglycine.-Plant Physiol. 97: 1221-1225, 1991. Go to original source...
    14. Matamoros, M.A., Baired, L.M., Escuredo, P.R., Dalton, D.A., Minchin, F.R., Itube-Ormaetxe, I., Rubio, M.C., Moran, J.F., Gordon, A.J., Becana, M.: Stress-induced legume root nodules senescence. Physiological, biochemical and structural alterations.-Plant Physiol. 121: 97-111, 1999. Go to original source...
    15. Miller, A.R., Pengelly, W.L.: Ethylene production by shoot forming and unorganised crown-gall tumor tissues of Nicotiana and Lycopersicon cultured in vitro.-Planta 161: 418-424, 1984. Go to original source...
    16. Morita, S., Kaminaka, H., Masumara, T., Tanaka, K.: Induction of rice cytosolic ascorbate peroxidase mRNA by oxidative stress: the involvement of hydrogen peroxide in oxidative stress signalling.-Plant Cell Physiol. 40: 417-422, 1999. Go to original source...
    17. Nandwal, A.S., Bharti, S., Singh, U.: Application of NO3-N and NH4 +-N affecting the leghemoglobin, N-status and dry matter production in chickpea.-Indian J. exp. Biol. 31: 93-95, 1993.
    18. Patterson, B.D., Machae, E.A., Ferguson, L.G.: Estimation of hydrogen peroxide in plant extracts using titanium (IV).-Anal. Biochem. 139: 487-492, 1984. Go to original source...
    19. Pladys, D., Barthe, P., Rigaud, J.: changes in intracellular pH in French bean nodules induced by senescence and nitrate treatment.-Plant Sci. 57: 99-106, 1988. Go to original source...
    20. Polle, A., Rennenberg, H.: Significance of antioxidants in plant adaptation to environmental stress.-In: Fowden, L., Mansfield, T., Stoddart, J. (ed.): Plant Adaptation to Environmental Stress. Pp. 263-273. Chapman and Hall, London 1993.
    21. Rawal, A., Nandwal, A.S., Kundu, B.S., Sheokand, S., Dayal, J.: Ethylene inhibitor restores changes in Cicer arietinum L. nodules induced by NO3-N.-Indian J. exp. Biol. 32: 732-735, 1994.
    22. Schmidt, J.S., Harper, J.E., Hoffman, T.K., Bent, A.F.: Regulation of soybean nodulation independent of ethylene signalling.-Plant Physiol. 119: 951-959, 1999. Go to original source...
    23. Smith, J.J., John, P.: Maximising the activity of the ethylene forming enzyme.-In: Peach, J.C., Latche, A., Balague, C. (ed.): Cellular and Molecular Aspects of the Plant Hormone Ethylene. Pp. 33-38. Kluwer Academic Publishers, Dordrecht 1993. Go to original source...
    24. Sullivan, C.Y.: Mechanism of heat and drought resistance in grain sorghum and methods of measurement.-In: Rao, N.G.P., House, L.R. (ed.): Sorghum in Seventies. Pp. 247-264. Oxford and IBH Publishing Co., New Delhi 1972.
    25. Vassileva, V., Ignatov, G.: Effect of high nitrate concentrations on dicarboxylate transport across the peribacteroid membrane of soybean root nodules.-J. Plant Physiol. 149: 222-224, 1996. Go to original source...
    26. Vessey, J.K., Waterer, J.-Search of the mechanism of nitrate inhibition of nitrogenase activity in legume nodules: recent developments.-Physiol. Plant. 84: 171-176, 1992. Go to original source...
    27. Yang, S.F., Hoffman, N.E.: Ethylene biosynthesis and its regulation in higher plants.-Annu. Rev. Plant Physiol. 35: 155-189, 1984. Go to original source...

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