biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 44:137-140, 2001 | DOI: 10.1023/A:1017903312036

An Electrophoretic Analysis of the Seed Protein Body Proteins from Pinus Nigra

M. Hajduch1, J. Nahálková2, J. Høib1, B. Vooková1, P. Gemeiner2
1 Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia
2 Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia

Protein bodies (PBs) of European black pine (Pinus nigra Arn.) were isolated from mature seeds. Extracted soluble matrix proteins and crystalloid proteins PBs proteins were investigated by SDS-PAGE electrophoresis in presence and absence of 2-mercaptoethanol. The proteins of molecular masses 16, 17, 18, 61 and 65 kDa were presented only in crystalloid protein samples. Only 15 kDa protein was present in soluble matrix proteins and not in crystalloid proteins. Another protein bands were present in both soluble matrix and crystalloid proteins. 20, 37, 38, 39 and 48 kDa proteins were strongly visible among crystalloid proteins. Bands of 23 and 32 kDa were more visible in soluble matrix protein samples. Different composition in crystalloid proteins was found in absence of 2-mercaptoethanol: no proteins with molecular mass 71 kDa and more proteins in soluble matrix. In case of crystalloid proteins we detected 7 protein bands in interval from 71 to 212 kDa.

Keywords: Pinus nigra; protein bodies; matrix and crystalloid proteins; SDS-PAGE
Subjects: crystalloid and matrix proteins, seed protein bodies; matrix and crystalloid proteins, seed protein bodies; pine; Pinus nigra; protein bodies; seed protein bodies, matrix and crystalloid proteins

Published: March 1, 2001  Show citation

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Hajduch, M., Nahálková, J., Høib, J., Vooková, B., & Gemeiner, P. (2001). An Electrophoretic Analysis of the Seed Protein Body Proteins from Pinus Nigra. Biologia plantarum, 44(1), 137-140. doi: 10.1023/A:1017903312036
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