biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:161-165, 2001 | DOI: 10.1023/A:1017982619635

Insertion Profiles in Stomatal Density and Sizes in Nicotiana Tabacum L. Plantlets

M. Voleníková1, I. Tichá2
1 Faculty of Science, Department of Plant Physiology, Charles University of Prague, Praha 2, Czech Republic
2 Faculty of Science, Department of Plant Physiology, Charles University of Prague, Praha 2, Czech Republic

Tobacco (Nicotiana tabacum L. cv. Samsun) plantlets were cultured in vitro on Murashige-Skoog medium photoautotrophically (without sucrose) or photomixotrophically (with 3 % sucrose) under two irradiances [70 or 230 µmol m-2 s-1]. Significant differences in stomatal density and sizes in leaves of different insertion levels (3rd, 5th and 7th leaves from bottom) in photomixotrophic plantlets but not in photoautotrophic ones were found after 35 d of culture. Stomatal density was higher in upper leaves and on abaxial leaf side. Higher irradiance enhanced stomatal density in photoautotrophic plantlets. Stomatal sizes decreased with leaf insertion level but no significant differences between leaf sides were found. Abaxial stomata were more circular than the adaxial ones. In photomixotrophic plantlets stomata tended to be more elongated in the 3rd and the 5th leaves, whereas stomatal elongation in photoautotrophic plantlets was similar in all leaves.

Keywords: irradiance; photoautotrophic and photomixotrophic growth; plant heterogeneity; stomatal elongation and shape; sucrose
Subjects: elongation, stomata, in vitro culture; heterogeneity of plant, stomata; in vitro culture, autotrophy development; in vitro culture, sugars as C source, low sugar media; insertion profile, stomata density and sizes; irradiance, stomata, in vitro culture; Nicotiana tabacum; photoautotrophy, in vitro culture; photomixotrophy, in vitro culture; stomata morphology, insertion profile; tobacco

Published: June 1, 2001  Show citation

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Voleníková, M., & Tichá, I. (2001). Insertion Profiles in Stomatal Density and Sizes in Nicotiana Tabacum L. Plantlets. Biologia plantarum, 44(2), 161-165. doi: 10.1023/A:1017982619635
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    References

    1. Burrows, G.E., Bullock, S.: Leaf anatomy of wollemi pine (Wollemia nobilis, Araucariaceae).-Aust. J. Bot. 47: 795-806, 1999. Go to original source...
    2. Čatský, J., Šesták, Z.: Photosynthesis during leaf ageing.-In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 633-660. Marcel Dekker, New York-Basel-Hong Kong 1997.
    3. De Pereira-Netto, A.B., Gabriele, A.C., Pinto, H.S.: Aspects of leaf anatomy of kudzu (Pueraria lobata, Leguminosae-Faboideae) related to water and energy balance.-Pesq. agropec. bras. 34: 1361-1365, 1999. Go to original source...
    4. Drew, A.P., Kavanagh, K.L., Maynard, C.A.: Acclimatizing micropropagated black cherry by comparison with half-sib seedlings.-Physiol. Plant. 86: 459-464, 1992. Go to original source...
    5. Furukawa, A.: Ontogenetic changes in stomatal size and conductance of sunflowers.-Ecol. Res. 7: 147-153, 1992. Go to original source...
    6. Furukawa, A.: Stomatal frequency of Quercus myrsinaefolia grown under different irradiances.-Photosynthetica 34: 195-199, 1997. Go to original source...
    7. Glater, R.B., Solberg, R.A., Scott, F.M.: A developmental study of the leaves of Nicotiana glutinosa as related to their smogsensitivity.-Amer. J. Bot. 49: 954-970, 1962. Go to original source...
    8. Lee, N., Wetzstein, H.Y., Sommer, H.E.: Quantum flux density effects on the anatomy and surface morphology of in vitro-and in vivo-developed sweetgum leaves.-J. amer. Soc. hort. Sci. 113: 167-171, 1988. Go to original source...
    9. Maximov, N.A.: The Plant in Relation to Water. A Study of the Physiological Basis of Drought Resistance.-George Allen and Unwin, London 1929.
    10. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. g. 15: 473-497, 1962. Go to original source...
    11. Noé, N., Bonini, L.: Leaf anatomy of highbush blueberry grown in vitro and acclimatization to ex vitro conditions.-Biol. Plant. 38: 19-25, 1996. Go to original source...
    12. Pierik, R.L.M.: In Vitro Culture of Higher Plants.-Kluwer Academic Publishers, Dordrecht-Boston-London 1997. Go to original source...
    13. Pospíšilová, J., Wilhelmová, N., Synková, H., Čatský, J., Krebs, D., Tichá, I., Hanáčková, B., Snopek, J.: Acclimation of tobacco plantlets to ex vitro conditions as affected by application of abscisic acid.-J. exp. Bot. 49: 863-869, 1998. Go to original source...
    14. Shackel, K.A., Novello, V., Sutter, E.G.: Stomatal function and cuticular conductance in whole tissue-cultured apple shoots.-J. amer. Soc. hort. Sci. 115: 468-472, 1990. Go to original source...
    15. Slavík, B.: The distribution pattern of transpiration rate, water saturation deficit, stomata number and size, photosynthetic and respiration rate in the area of the tobacco leaf blade.-Biol. Plant. 5: 143-153, 1963. Go to original source...
    16. Sutter, E.: Stomatal and cuticular water loss from apple, cherry and sweetgum plants after removal from in vitro culture.-J. amer. Soc. hort. Sci. 113: 234-238, 1988. Go to original source...
    17. Tichá, I.: Photosynthetic characteristics during ontogenesis of leaves: 7. Stomata density and sizes.-Photosynthetica 16: 375-471, 1982.
    18. Tichá, I.: Ontogeny of leaf morphology and anatomy.-In: Šesták, Z. (ed.): Photosynthesis During Leaf Development. Pp. 16-50. Academia, Praha 1985. Go to original source...
    19. Tichá, I.: Optimization of photoautotrophic tobacco in vitro culture: effect of suncaps closures on plantlet growth.-Photosynthetica 32: 475-479, 1996.
    20. Tichá, I., Obermayer, P., Snopek, J.: Stomata density and sizes in in vitro grown tobacco plantlets.-Acta Fac. Rer. nat. Univ. Comenianae-Physiol. Plant. 29: 101-107, 1997.
    21. Tichá, I., Radochová, B., Kadleček, P.: Stomatal morphology during acclimatization of tobacco plantlets to ex vitro conditions.-Biol. Plant. 42: 469-474, 1999. Go to original source...
    22. Tichá, I., Čatský, J., Hodáňová, D., Pospišilová, J., Kaše, M., Šesták, Z.: Gas exchange and dry matter accumulation during leaf development.-In: Šesták, Z. (ed.): Photosynthesis During Leaf Development. Pp. 157-216. Academia, Praha 1985. Go to original source...
    23. Tichá, I., Čáp, F., Pacovská, D., Hofman, P., Haisel, D., Čapková, V., Schäfer, C.: Culture on sugar medium enhances photosynthetic capacity and high light resistance of plantlets grown in vitro.-Physiol. Plant. 102: 155-162, 1998. Go to original source...
    24. Turner, N.C., Begg, J.E.: Stomatal behavior and water status of maize, sorghum, and tobacco under field conditions.-Plant Physiol. 51: 31-36, 1973. Go to original source...
    25. Wetzstein, H.Y., Sommer, H.E.: Scanning electron microscopy of in vitro-cultured Liquidambar styraciflua plantlets during acclimatization.-J. amer. Soc. hort. Sci. 108: 475-480, 1983. Go to original source...
    26. Weyers, J.D.B., Meidner, H.: Methods in Stomatal Research.-Longman Scientific & Technical, Esses 1990.
    27. Weyers, J.D.B., Lawson, T.: Heterogeneity in stomatal characteristics.-Adv. bot. Res. 26: 317-352, 1997. Go to original source...
    28. Wild, A., Wolf, G.: The effect of different light intensities on the frequency and size of stomata, the size of cells, the number, size and chlorophyll content of chloroplasts in the mesophyll and the guard cells during the ontogeny of primary leaves of Sinapis alba.-Z. Pflanzenphysiol. 97: 325-342, 1980. Go to original source...
    29. Zacchini, M., Morini, S., Vitagliano, C.: Effect of photoperiod on some stomatal characteristics of in vitro cultured fruit tree shoots.-Plant Cell Tissue Organ Cult. 49: 195-200, 1997. Go to original source...
    30. Zalenskiï, V. R.: [Materials concerning the quantitative anatomy of different leaves of one and the same plant.]-Mem. polytekh. Inst. (Kiev) 4: 1-203, 1904. [In Russ.]
    31. Ziv, M., Ariel, T.: Vitrification in relation to stomatal deformation and malfunction in carnation leaves in vitro.-In: Lumsden, P.J., Nicholas, J.R., Davies, W.J. (ed.): Physiology, Growth and Development of Plants in Culture. Pp. 143-154. Kluwer Academic Publishers, Dordrecht-Boston-London 1994. Go to original source...

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