biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:185-188, 2001 | DOI: 10.1023/A:1010230821452

Effect of Irradiance, Sugars and Nitrogen on Leaf Size of In Vitro Grown Ceratonia Siliqua L.

B. Vinterhalter1, D. Vinterhalter1, M. Nešković1
1 Plant Physiology Department, Institute for Biological Research "Siniša Sanković", Belgrade, Yugoslavia

Carob (Ceratonia siliqua L.) has compound pinnate leaves consisting of 4 - 6 pairs of leaflets. However, in conditions of in vitro culture only one pair of leaflets develops. With increasing irradiance from 9.3 to 74.1 µmol m-2 s-1, leaf area increased 5-fold. Sucrose also significantly increased leaf area and the maxima were at concentration 147 mM at high irradiance and 233.6 mM at low irradiance. Sucrose was superior to fructose, glucose and combination of both in increasing leaf area. Decreasing concentration of KNO3 and NH4NO3 caused a 3-fold decline of leaf area.

Keywords: carob; leaf area; sucrose; fructose; glucose
Subjects: carrob; Ceratonia siliqua; in vitro culture, sugars as C source, low sugar media; irradiance, leaf size in vitro; nitrogen supply, structure and ultrastructure; photoautotrophy, in vitro culture; photomixotrophy, in vitro culture; sugars, leaf size in vitro

Published: June 1, 2001  Show citation

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Vinterhalter, B., Vinterhalter, D., & Nešković, M. (2001). Effect of Irradiance, Sugars and Nitrogen on Leaf Size of In Vitro Grown Ceratonia Siliqua L. Biologia plantarum, 44(2), 185-188. doi: 10.1023/A:1010230821452
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    References

    1. Aksenova, N.P., Konstatinova, T.N., Sergeeva, L.I., Macháčková, I., Golyanovskaya, S.A.: Morphogenesis of potato plants in vitro. I. Effect of light quality and hormones.-J. Plant Growth Regul. 13: 143-146, 1994. Go to original source...
    2. Alorda, M., Medrano, H.: Micropropagation of carob (Ceratonia siliqua L.) from shoot buds of young plants.-Proc. 2nd Congr. Nat. Soc. Hort. Sci. Vol. II, Pp. 919-924. Cordoba 1986. [In Span.]
    3. Batlle, I., Tous, J.: Carob Tree, Ceratonia siliqua L. (Promoting the Conservation and Use of Underutilized and Neglected Crops. Vol. 17.)-Institute of Plant Genetics and Crop Plant Research, Gatersleben; International Plant Genetic Resources Institute, Rome 1997.
    4. Dale, J.E.: The control of leaf expansion.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 267-295, 1988. Go to original source...
    5. Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.-Exp. Cell Res. 50: 151-158, 1968. Go to original source...
    6. Kitaya, Y., Fukuda, O., Kozai, C., Kirdmanee, C.: Effects of light intensity and lighting direction on the photoautotrophic growth and morphology of potato plantlets in vitro.-Scientia Hort. 62: 15-24, 1995. Go to original source...
    7. Lloyd, G., McCown, B.: Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture.-Proc. int. Plant Propag. Soc. 30: 421-427, 1981.
    8. Marks, T.R., Simpson, S.E.: Effect of irradiance on shoot development in vitro.-Plant Growth Regul. 28: 133-142, 1999. Go to original source...
    9. Moreira de Silva, M.H., Debergh, P.C.: The effect of light quality on the morphogenesis of in vitro cultures of Azorina vidalii (Wats.) Feer.-Plant Cell Tissue Organ Cult. 51: 187-193, 1997. Go to original source...
    10. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    11. Saebo, A., Krekling, T., Appelgren, M.: Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro.-Plant Cell Tissue Organ Cult. 41: 177-185, 1995. Go to original source...
    12. Sebastian, K.T., McComb, J.A.: A micropropagation system for carob (Ceratonia siliqua L).-Scientia Hort. 28: 127-131, 1986. Go to original source...
    13. Smith, M.A., Palta, J.P., McCown, B.H.: Comparative anatomy and physiology of microcultured, seedling and greenhouse grown Asian white birch.-J. amer. Soc. hort. Sci. 111: 437-442, 1986. Go to original source...
    14. Thomas, V., Mehta, A.R.: Effect of phloroglucinol on shoot growth and initiation of roots in carob tree cultures grown In vitro.-In: Sen, S.K., Giles, L.K., (ed.): Plant Cell Culture in Crop Improvement. Pp 451-457. Plenum Press, New York-London 1983. Go to original source...
    15. Vinterhalter, B.: Effect of light and sucrose on growth and structure of carob (Ceratonia siliqua L.) shoot cultures.-PhD Thesis, Belgrade University, Belgrade 1998.
    16. Vinterhalter, D., Vinterhalter, B.: Factors affecting in vitro propagation of carob (Ceratonia siliqua L.).-Arch. biol. Sci. Belgrade 44: 177-186, 1992.
    17. Vinterhalter, D., Vinterhalter, B.: Hormone-like effects of sucrose in plant in vitro cultures.-Phyton 39: 57-60, 1999.

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