biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 44:225-232, 2001 | DOI: 10.1023/A:1010247325086

Free Radical Scavengers and Photosynthetic Pigments in Pinus Cembra L. Needles as Affected by Ozone Exposure

G. Wieser1, M. Tausz2, A. Wonisch2, W.M. Havranek1
1 Forstliche Bundesversuchsanstalt, Institut für Immissionsforschung und Forstchemie, Abteilung für Forstpflanzenphysiologie, Innsbruck, Austria
2 Institut für Pflanzenphysiologie, Karl-Franzens-Universität Graz, Graz, Austria

The goal of this study was the characterization of the antioxidative protection system of current and 1-year-old needles of a cembran pine (Pinus cembra L.) and its possible responses to elevated concentrations of atmospheric O3. Twigs of a mature cembran pine at the alpine timberline (1950 m a.s.l.) were exposed in climate-controlled twig chambers for 91 d to charcoal-filtered air (CF), ambient air O3 concentration (A), and two-fold ambient air O3 concentration (2A). Additionally, a chamberless control group (AA) was used to examine chamber effects. At the end of the fumigation period the contents of free radical scavengers and photosynthetic pigments were measured in the needles. Independent from O3 exposure, total ascorbate and α-tocopherol contents were higher in 1-year-old needles compared to the current flush while the opposite was found for glutathione. The amounts of pigments and antioxidants in P. cembra needles were comparable to those in other conifers growing at high-elevation sites. The only hint toward O3 induced changes in the composition of antioxidants was an increase in the glutathione redox state toward more oxidation in 1-year-old needles upon exposure to A or AA conditions, but not upon 2A exposure. Chlorophyll and carotenoid contents were not affected by O3 neither in current- nor in previous-year needles. The de-epoxidation state of the xanthophyll cycle pigments, however, was significantly increased in 1-year-old needles under A and AA compared to the CF control, but not under 2A. Hence, Pinus cembra, which is well adapted to the extreme environment of the timberline ecotone, exhibited only marginal biochemical changes in response to elevated O3.

Keywords: ascorbic acid; cembran pine; glutathione; oxidative stress; stress; xanthophyll cycle
Subjects: cembran pine; chloroplast pigments, free radical scavengers, ozone; free radical scavengers, photosynthetic pigments, ozone; ozone, free radical scavengers; pine; Pinus cembra; xanthophyll cycle

Published: June 1, 2001  Show citation

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Wieser, G., Tausz, M., Wonisch, A., & Havranek, W.M. (2001). Free Radical Scavengers and Photosynthetic Pigments in Pinus Cembra L. Needles as Affected by Ozone Exposure. Biologia plantarum, 44(2), 225-232. doi: 10.1023/A:1010247325086
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