biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:283-288, 2001 | DOI: 10.1023/A:1010263728721

Endopeptidases of Triticale Seeds

W. Bielawski1, B. Prabucka1
1 Department of Biochemistry, Warsaw Agricultural University, Warszawa, Poland

The changes in endopeptidase activity in different parts of germinating triticale cv. Malno were investigated. Haemoglobin, gliadin, azocasein and azoalbumin were used as substrates. During the first day of germination the activity of haemoglobin hydrolyzing endopeptidases predominated while after the second day, mainly in the endosperm, a rapid increase in endopeptidases activity preferring gliadin hydrolysis was observed. In all the investigated tissues azocaseinolytic activities increased with the successive days of germination. Similar changes were observed using azoalbumin with one exception: in the embryo axis this activity decreased with the progression of germination. Separation of endopeptidases on the DEAE Sepharose CL-6B reveals three activity peaks in extract from dry seeds and four peaks in extract from 3 d germinated seeds. The obtained peaks differed in substrate specificity and in sensitivities to class-specific inhibitors.

Keywords: aspartyl; cysteine; hydrolytic specificity; serine endopeptidases; Triticosecale
Subjects: endopeptidases, triticale; seed endopeptidases, triticale; triticale, Triticosecale

Published: June 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Bielawski, W., & Prabucka, B. (2001). Endopeptidases of Triticale Seeds. Biologia plantarum, 44(2), 283-288. doi: 10.1023/A:1010263728721
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Belozersky, M., Sarbakanowa, Sh.T., Dunaevsky, Ya.E.: Aspartic proteinase from wheat seeds: isolation, properties and action on gliadin.-Planta 177: 321-326, 1989. Go to original source...
    2. Bethke, P.C., Hillmer, S., Jones, R.J.: Isolation of intact protein storage vacuoles from barley aleurone.-Plant Physiol. 110: 521-529, 1996. Go to original source...
    3. Bethke, P.C., Swanson, S.J., Hillmer, S., Jones, R.L.: From storage compartment to lytic organelle: the metamorphosis of the aleurone protein storage vacuole.-Ann. Bot. 82: 399-412, 1998. Go to original source...
    4. Bielawski, W., Dojczew, D., Kączkowski, J., Kolbuszewska-Podres, W.: Enzymes of protein breakdown in germinating triticale grains resistant and susceptible to pre-harvest sprouting.-Acta Physiol. Plant. 16: 19-26, 1994.
    5. Bottari, A., Capocchi, A., Fontanini, D., Galleschi, L.: Major proteinase hydrolysing gliadin during wheat germination.-Phytochemistry 43: 39-44, 1996. Go to original source...
    6. Casano, L., Desimone, M., Trippi, V.S.: Proteolytic activity at alkaline pH in oat leaves, isolation of an aminopeptidase.-Plant Physiol. 91: 1414-1418, 1989. Go to original source...
    7. Fincher, G.B.: Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains.-Annu. Rev. Plant Physiol. Plant mol. Biol. 40: 305-346, 1989. Go to original source...
    8. Galleschi, L., Capocchi, A., Giannoni, P., Floris, C.: Proteinase activities in quiescent and germinating seeds of ×Hyanaldoticum sardoum.-Physiol. Plant. 75: 1-6, 1989. Go to original source...
    9. Jacobsen, J.V., Varner, J.E.: Gibberellic acid-induced synthesis of protease by isolated aleuron layers of barley.-Plant Physiol. 42: 1596-1600, 1967. Go to original source...
    10. Kervinen, J., Sarkkinen, P., Mikola, L., Saarma, M.: Hydrolytic specificity of the barley grain aspartic proteinase.-Phytochemistry 32: 799-803, 1993. Go to original source...
    11. Kolaczkowska, M. K., Wieczorek, M., Polanowski, A.: An aspartic proteinase from Fusarium moniliforme.-Eur. J. Biochem. 132: 557-561, 1983. Go to original source...
    12. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    13. Marttila, S., Jones, B.L., Mikkonen, A.: Differential localization of two acid proteinases in germinating barley (Hordeum vulgare) seed.-Physiol. Plant. 93: 317-327, 1995. Go to original source...
    14. Mikkonen, A., Porali, I., Cercos, M., Ho, T-h.D.: A major cysteine proteinase, EPB, in germinating barley seeds: structure of two intronless genes and regulation of expression.-Plant mol. Biol. 31: 239-254, 1996. Go to original source...
    15. Miller, B.L., Huffaker, R.C.: Partial purification and characterization of endoproteinases from senescing barley leaves.-Plant Physiol. 68: 930-936, 1981. Go to original source...
    16. Multu, A., Gal, S.: Plant aspatric proteinases: enzymes on the way to a function.-Physiol. Plant. 105: 569-576, 1999. Go to original source...
    17. Nguyen, C.V., Bielawski, W., Kączkowski, J.: Distribution of endopeptidases in germinating triticale grains susceptible and resistant to pre-harvest sprouting.-Acta Physiol. Plant. 17: 9-16, 1995a.
    18. Nguyen, C.V., Bielawski, W., Kączkowski, J.: Peptidase activity changes in maturing triticale grains susceptible and resistant to pre-harvest sprouting.-Acta Physiol. Plant. 17: 241-248, 1995b.
    19. Shutov, A.B., Waintraub, I.A.: Degradation of storage proteins in germinating seeds.-Phytochemistry 26: 1557-1566, 1987. Go to original source...
    20. Voigt, G., Biehl, B., Heinrichs, H., Voigt, J.: Aspartic proteinase levels in seeds of different angiosperms.-Phytochemistry 44: 389-392, 1997. Go to original source...
    21. Zhang, N., Jones, B.L.: Purification and partial characterization of a 31-kDa cysteine endopeptidase from germinated barley.-Planta 199: 565-572, 1996. Go to original source...

    Return to the content