biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:333-337, 2001 | DOI: 10.1023/A:1012422222423

Shoot Regeneration from Immature Cotyledons of Cicer Arietinum

K. Srivastava1, K.N. Tiwari1, R. Singh1, B.D. Singh1, H.K. Jaiswal2
1 School of Biotechnology, Faculty of Science, B.H.U, Varanasi-, India
2 Institute of Agricultural Sciences, B.H.U., Varanasi-, India

Shoot regeneration was achieved from immature cotyledons of five chickpea (Cicer arietinum L.) genotypes: C235, ICC4971, ICC11531, ICC12257 and ICC12873. The cotyledons cultured on Murashige and Skoog (MS) medium supplemented with 3 or 5 mg dm-3 zeatin with or without 0.04 mg dm-3 indole acetic acid (IAA) showed formation of cotyledon like structures (CLS) at their proximal ends. Subsequently, shoot regeneration took place in some of the CLS forming explants. CLS were also formed in cotyledons cultured on MS + 0.2 - 1 mg dm-3 thidiazuron (TDZ); direct shoot regeneration was observed in cotyledons cultured on 1 mg dm-3 TDZ. The shoot buds elongated on media containing indole butyric acid (IBA), benzylaminopurine (BAP) and gibberellic acid (GA3). Complete plantlets were obtained by rooting of shoots following pulse treatment with 200 mg dm-3 IBA for 5 min and culture on growth regulator free half-strength MS medium.

Keywords: chickpea; cotyledon like structures; regeneration in vitro; thidiazuron; zeatin
Subjects: auxins; chickpea; Cicer arietinum; cotyledon-like structures, shoot regeneration in vitro; cytokinins; gibberellins; in vitro culture, micropropagation, regeneration; thidiazuron, shoot regeneration; zeatin, shoot regeneration from immature cotyledons

Published: September 1, 2001  Show citation

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Srivastava, K., Tiwari, K.N., Singh, R., Singh, B.D., & Jaiswal, H.K. (2001). Shoot Regeneration from Immature Cotyledons of Cicer Arietinum. Biologia plantarum, 44(3), 333-337. doi: 10.1023/A:1012422222423
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