Biologia plantarum 44:355-360, 2001 | DOI: 10.1023/A:1012430408310
Effects of Benzylaminopurine and Abscisic Acid on Distribution of rRNA in the Palisade Cells of Excised Cucurbita Pepo Cotyledons
- 1 Institute of Plant Physiology "Acad. M. Popov", Bulgarian Academy of Sciences, Sofia, Bulgaria
rRNA was labelled with RNase-gold complexes applied to ultrathin Lowicryl sections of excised Cucurbita pepo L. (zucchini) cotyledons grown in darkness. Benzylaminopurine-caused stimulation of cotyledon growth decreases the density of ribosomes in palisade cells despite stimulating rRNA synthesis. Abscisic acid inhibits RNA synthesis and transport, but does not visibly affect the number of pre-existing ribosomes in retarded growth cells. The amount of rRNA in the cells of 24 h treated cotyledons is rather consequence of the growth rate than its leading factor.
Keywords: nucleolus; rRNA synthesis and transport; cell ultrastructure; ribosomes; morphometry; cell growth
Subjects: abscisic acid; cell ultrastructure, cotyledon rRNA distribution in palisade cells; cotyledon cell ultrastructure, rRNA distribution in palisade cells; Cucurbita pepo; cytokinins; palisade cells, rRNA distribution; ribosome, rRNA distribution in palisade cells, cotyledon; rRNA distribution in palisade cells, cotyledon; zuccini
Published: September 1, 2001 Show citation
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Stoynova-Bakalova, E.Z., Petrov, P.I., & Karanov, E.N. (2001). Effects of Benzylaminopurine and Abscisic Acid on Distribution of rRNA in the Palisade Cells of Excised Cucurbita Pepo Cotyledons. Biologia plantarum, 44(3), 355-360. doi: 10.1023/A:1012430408310
References
- Ananiev, E.D., Karagyozov, L.K., Karanov, E.N.: Effect of cytokinins on ribosomal RNA gene expression in excised cotyledons of Cucurbita pepo L.-Planta 170: 370-378, 1987.
Go to original source... - Ananieva, K.I., Ananiev, E.D.: Effect of methyl ester of jasmonic acid and benzylaminopurine on growth and protein profile of excised cotyledons of Cucurbita pepo (zucchini).-Biol. Plant. 42: 549-557, 1999.
Go to original source... - Banerji, D., Laloraya, M.M.: Expansion of isolated pumpkin cotyledons with kinetin: effect of seedling age.-Naturwissenschaften 52: 349-350, 1965.
Go to original source... - Bendayan, M.: Ultrastructural localisation of nucleic acids by the use of enzyme-gold complexes: influence of fixation and embedding.-Biol. Cell 43: 153-156, 1982.
- Bewli, I.S., Witham, F.H.: Characterization of the kinetin-induced water uptake by detached radish cotyledons.-Bot. Gaz. 137: 58-64, 1976.
Go to original source... - Charest, P., Bergeron, F., Lafontaine, J.: Ultrastructural localisation of DNA and RNA in Allium porrum interphase cells by means of nuclease-gold complexes.-Histochem. J. 17: 957-966, 1985.
Go to original source... - Cheniclet, C., Carde, J.P.: Ultrastructural localisation of RNAs in plastids with an RNase-gold method.-Biol. Cell 59: 79-88, 1987.
- Fofanova, T.A., Khokhlova, V.A.: [Tissue specificity of the cell response to phytohormones in excised pumpkin cotyledons.]-Fiziol. Rast. 30: 421-430, 1983. [In Russ.]
- Frens, G.: Controlled nucleation for the regulation of the particle size in monodisperse gold solutions.-Nature 241: 20-21, 1973.
Go to original source... - Gordon, E.M., Letham, D.S.: Regulators of cell division in plant tissues. XXII. Physiological aspects of cytokinin-induced radish cotyledon growth.-Aust. J. Plant Physiol. 2: 129-154, 1975.
Go to original source... - Hadjiolov, A.A.: The Nucleolus and Ribosome Biogenesis.-Springer-Verlag, Vienna 1985.
Go to original source... - Harvey, B.M., Lu, B.C., Fletcher, R.A.: Benzyladenine accelerates chloroplast differentiation and stimulates photosynthetic enzyme activity in cucumber cotyledons.-Can. J. Bot. 52: 2581-2586, 1974.
Go to original source... - Ho, D.T., Varner, J.E.: Response of barley aleurone layers to abscisic acid.-Plant Physiol. 57: 175-178, 1976.
Go to original source... - Ihle, J.N., Dure, L.: Hormonal regulation of translation inhibition requiring RNA synthesis.-Biochem. biophys. Res. Commun. 38: 995-1001, 1970.
Go to original source... - Klyachko, N.L., Ananiev, E., Kulaeva, O.N.: Effect of 6-benzylaminopurine and abscisic acid on protein synthesis in isolated pumpkin cotyledons.-Physiol. vég. 17: 607-617, 1979.
- Kulaeva, O.N.: Tsytokininy, ikh Struktura i Funktsiya. [Cytokinins, their structure and function.]-Nauka, Moskva 1973. [In Russ.]
- Leshem, Y., Schwarz, L.: The selective effect of abscisic acid on ribonucleic acid components.-Physiol. Plant. 26: 328-331, 1972.
Go to original source... - Letham, D.S.: Regulators of cell division in plant tissues. XII. A cytokinin bioassay using excised radish cotyledons.-Physiol. Plant. 25: 391-396, 1971.
Go to original source... - Mikulovich, T.P., Wollgiehn, R., Khokhlova, V.A., Neumann, D., Kulaeva, O.N.: Synthesis of plastid and cytoplasmic rRNAs in isolated pumpkin cotyledons. II. Effect of cytokinin and light.-Biochem. Physiol. Pflanz. 172: 101-110, 1978.
Go to original source... - Milborrow, B.V.: The chemistry and physiology of abscisic acid.-Annu. Rev. Plant Physiol. 25: 195-307, 1974.
Go to original source... - Murai, N.: Cytokinin biosynthesis in tRNA and cytokinin incorporation into plant RNA.-In: Mok, D.W.S., Mok, M.C. (ed.): Cytokinins. Chemistry, Activity, and Function. Pp. 233-242. CRC Press, Boca Raton 1994.
- Naito, K., Ikeda K., Saito, T., Suzuki, H., Yamada, M., Kuraishi, S.: Importance of RNA synthesis within the lag phase proceeding benzyladenine-induced growth of cucumber cotyledons in the dark.-Plant Cell Physiol. 21: 1419-1430, 1980.
Go to original source... - Piche, Y., Peterson, R.L., Ackerley, C.A., Rauser, W.E.: RNase-gold labelling in primary roots of Zea mays L.; evaluation of a particulate marker.-Plant Sci. Lett. 36: 155-160, 1984.
Go to original source... - Pilet, P.E.: ABA effects on growth in relation to auxin, RNA and ultrastructure.-In: Kaldeway, H., Vardar, Y. (ed.): Hormonal Regulation in Plant Growth and Development. Pp. 297-315. Springer-Verlag, Vienna 1972.
- Roussaux, J., Hoffelt, M., Farineau, N.: Evolution des RNA ribosomaux au cours du verdissement de cotyledons de concombre en presence de 6-benzylaminopurine.-Can. J. Bot. 54: 2328-2336, 1976.
Go to original source... - Sato, S.: Estimation of RNA content of the nucleolus and nucleolus-derived structures in Vicia faba mitotic cells embedded in Lowicryl resin using RNase-gold labelling.-J. Electron. Microscop. 39: 382-387, 1990.
- Sato, S.: Tree-dimensional architecture of the higher plant nucleolonema disclosed on scrial ultrathin sections.-Biol. Cell 75: 225-233, 1992.
Go to original source... - Sato, S., Willson, C., Dickinson, H.G.: Origin of nucleolus-like bodies found in the nucleoplasm of Vicia faba meristematic cells.-Biol. Cell 64: 321-329, 1988.
Go to original source... - Sato, S., Willson, C., Dickinson, H.G.: The RNA content of the nucleolus and nucleolus-like inclusions of the anther of Lilium estimated by an improved RNase-gold labelling method.-J. Cell Sci. 94: 675-683, 1989.
Go to original source... - Selivankina, S.Yu., Romanko, E.G., Kuroedov, V.A., Omann, E.: [Effect of cytokinins on RNA synthesis in etiolated rye seedlings.]-Fiziol. Rast. 23: 1011-1017, 1976. [In Russ.]
- Shen, Q., Ho, T.-H.D.: Promoter switches specific for abscisic acid-induced gene expression in cereals.-Physiol. Plant. 101: 653-664, 1997.
Go to original source... - Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy.-J. Ultrastruct. Res. 26: 31-43, 1969.
Go to original source... - Sussex, J., Clutter, M., Walbot, V.: Benzyladenine reversal of abscisic acid inhibition of growth and RNA synthesis in germinating been axes.-Plant Physiol. 56: 575-578, 1975.
Go to original source... - Trewavas, A.: The turnover of nucleic acids in Lemna minor.-Plant Physiol. 45: 742-751, 1980.
Go to original source... - Wareing, P.F., Good, J., Manuel, J.: Some possible physiological roles of abscisic acid.-In: Wightmant, F., Setterfield, G. (ed.): Biochemistry and Physiology of Plant Growth Substances. Pp. 1561-1579. The Running Press, Ottawa 1968.
- Zeevaart, J.A.D., Cleelman, R.A.: Metabolism and physiology of abscisic acid.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 439-473, 1988.
Go to original source...



