biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:447-450, 2001 | DOI: 10.1023/A:1012492105101

Activities of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Phosphoenolpyruvate Carboxylase, and Oxygen Evolution in Transgenic Tobacco Plants

J. Květoň1
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, and Faculty of Biological Sciences, University of South Bohemia, České Budějovice, Czech Republic

Three clones of tobacco transformed with the T-DNA of Agrobacterium rhizogenes Ri plasmid A4b cultivated in vitro on a solid agar medium neither showed pronounced morphological diversity nor significantly differed in chlorophyll (Chl) contents from control plants. However, the transformation caused a 27 to 83 % decay in leaf oxygen evolution and in both ribulose-1,5-bisphosphate carboxylase (RuBPC) and phosphoenolpyruvate carboxylase (PEPC) activities. Therefore, the transformation brought about a reduction of active PEPC as well as activable RuBPC amounts in plant tissues. Individual clones did not mutually differ. In tobacco transformed with A. rhizogenes strain TR101 and grown in soil only the mean leaf area tended to reduce. Chl contents, Chl a/b ratio, oxygen evolution, and activities of both RuBPC and PEPC were insignificantly affected by the transformation.

Keywords: Agrobacterium rhizogenes; Nicotiana tabacum; Ri plasmids. T-DNA; young; mature and old leaves
Subjects: Agrobacterium rhizogenes; chlorophylls, transgenic plants; leaf age, photosynthesis, transgenic plants; Nicotiana tabacum; ontogeny of leaf, photosynthesis; phosphoenolpyruvate carboxylase, transgenic plants; ribulose-1,5-bisphosphate carboxylase/oxygenase, oxygen evolution, transgenic plants; Ri-plasmids, photosynthetic traits; tobacco

Published: September 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Květoň, J. (2001). Activities of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Phosphoenolpyruvate Carboxylase, and Oxygen Evolution in Transgenic Tobacco Plants. Biologia plantarum, 44(3), 447-450. doi: 10.1023/A:1012492105101
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Binns, A.N.: Host and T-DNA determinants of cytokinin autonomy in tobacco cells transformed by Agrobacterium tumefaciens.-Planta 158: 272-279, 1983. Go to original source...
    3. Dehio, C., Grossmann, K., Schell, J., Schmülling, T.: Phenotype and hormonal status of transgenic tobacco plants overexpressing the rolA gene of Agrobacterium rhizogenes T-DNA.-Plant mol. Biol. 23: 1199-1210, 1993. Go to original source...
    4. Estruch, J.J., Schell, J., Spena, A.: The protein encoded by the rolB plant oncogene hydrolyses indole glucosides.-EMBO J. 10: 3125-3128, 1991. Go to original source...
    5. Filetici, P., Spanò, L., Costantino, P.: Conserved regions in the T-DNA of different Agrobacterium rhizogenes root inducing plasmids.-Plant mol. Biol. 9: 19-26, 1987. Go to original source...
    6. Genoud-Gourichon, C., Sallanon, H., Coudret, A.: Effects of sucrose, agar, irradiance and CO2 concentration during the routing phase on the acclimation of Rosa hybrida plantlets to ex vitro conditions.-Photosynthetica 32: 263-270, 1996.
    7. Lane, D., Maruyama, H., Easterday, R.L.: Phosphoenolpyruvate carboxylase from peanut cotyledons.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 13. Pp. 277-283. Academic Press, New York-London 1969. Go to original source...
    8. Lilley, R.M.C., Walker, D.A.: An improved spectrophotometric assay for ribulosebisphosphate carboxylase.-Biochim. biophys. Acta 358: 226-229, 1974. Go to original source...
    9. Nilsson, O., Moritz, T., Imbault, N., Sandberg, G., Olsson, O.: Hormonal characterization of transgenic tobacco plants expressing the rolC gene of Agrobacterium rhizogenes TL-DNA.-Plant Physiol. 102: 363-371, 1993. Go to original source...
    10. Offringa, I.A., Melchers, L.S., Regensburg-Tuink, A.J.G., Costantino, P., Schilperoort, R.A., Hooykaas, P.J.J.: Complementation of Agrobacterium tumefaciens tumor-inducing aux mutants by genes from the TR-region of the Ri plasmid of Agrobacterium rhizogenes.-Proc. nat. Acad. Sci. USA 83: 6935-6939, 1986. Go to original source...
    11. Ooms, G., Atkinson, J., Bossen, M.E., Leigh, R.A.: TL-DNA from Agrobacterium rhizogenes plasmid pRi1855 reduces the osmotic pressure in transformed plants grown in vitro.-Planta 168: 106-112, 1986. Go to original source...
    12. Petit, A., David, C., Dahl, G.A., Ellis, J.G., Guyon, P., Case-Delbart, F., Tempé, J.: Further extension of the opine concept: Plasmids in Agrobacterium rhizogenes cooperate for opine degradation.-Mol. gen. Genet. 190: 204-214, 1983. Go to original source...
    13. Pospíšilová, J., Tichá, I., Čatský, J., Solárová, J., Ondřej, M.: Photosynthesis in regenerants of tobacco transformed by plasmids of Agrobacterium. 3. Transplanted plants.-Photosynthetica 23: 130-135, 1989.
    14. Sallanon, H., Dimon, B., Carrier, P., Chagvardieff, P.: Effects of CO2 concentration and irradiance on growth and photosynthesis of Juglans regia plantlets grown in vitro.-Photosynthetica 31: 241-249, 1995.
    15. Šantrůček, J., Šiffel, P., Polišenská, M., Pešková, H.: Species-specific response of the photosynthetic apparatus of tobacco and potato to low CO2 concentrations.-Photosynthetica 25: 203-209, 1991.
    16. Schmülling, T., Fladung, M., Grossmann, K., Schell, J.: Hormonal content and sensitivity of transgenic tobacco and potato plants expressing single rol genes of Agrobacterium rhizogenes T-DNA.-Plant J. 3: 371-382, 1993. Go to original source...
    17. Šiffel, P., Durchan, M., Květoň, J., Ondřej, M., Šesták, Z.: Photosynthesis in regenerants of tobacco transformed by plasmids of Agrobacterium. 2. Fluorescence emission spectra and age induced changes in slow fluorescence induction.-Photosynthetica 22: 214-220, 1988.
    18. Tichá, I., Ondřej, M., Hrouda, M., Čatský, J., Zima, J., Pospíšilová, J., Solárová, J.: Photosynthesis in regenerants of tobacco transformed by plasmids of Agrobacterium. I. Morphology, anatomy and gas exchange of transformants.-Photosynthetica 22: 350-363, 1988.
    19. Václavík, J., Konečná, A., Ondřej, M.: Effect of pRi T-DNA transformation on growth and gas exchange of transplanted tobacco regenerants of the T and T' phenotypes.-Biol. Plant. 30: 432-439, 1988. Go to original source...

    Return to the content