biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 44:451-453, 2001 | DOI: 10.1023/A:1012496221939

Growth and Endogenous Cytokinins in Tobacco Callus as Affected by N-(2-chloro-4-pyridyl)-N'-phenylurea

L. Atanasova1, L. Iliev1
1 Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

The effect of N-(2-chloro-4-pyridyl)-N'-phenylurea (4PU-30) on the growth and content of endogenous cytokinins of adenine type in tobacco (Nicotiana tabacum L.) callus was investigated. Biomass accumulation in calli grown on Murashige and Skoog (MS) medium with 4PU-30 was higher than that on MS medium with kinetin. The obvious presence of isopentenyladenine type cytokinins and traces of zeatin type cytokinins supposes modification in the endogenous cytokinin metabolism of the tobacco callus grown on 4PU-30.

Keywords: Nicotiana labacum L.; phenylurea-adapted callus; endogenous adenine type cytokinins; indirect competitive ELISA
Subjects: cytokinins; in vitro culture, phenylurea-adapted callus; N-(2-chloro-4-pyridyl)-N'-phenylurea, 4PU-30; Nicotiana tabacum

Published: September 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Atanasova, L., & Iliev, L. (2001). Growth and Endogenous Cytokinins in Tobacco Callus as Affected by N-(2-chloro-4-pyridyl)-N'-phenylurea. Biologia plantarum, 44(3), 451-453. doi: 10.1023/A:1012496221939
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Armstrong, D.J.: Cytokinin oxidase and the regulation of cytokinin degradation-In: Mok, D.W.S., Mok, M.C. (ed.): Cytokinins, Chemistry, Activity and Function. Pp. 139-154. CRC Press, Boca Raton 1994. Go to original source...
    2. Atanassova, L.Y., Pissarska, M.G., Stoyanov, I.G.: Cytokinins and growth response of maize and pea plants to salt stress-Bulg. J. Plant Physiol. 22: 22-31, 1996.
    3. Burrows, W.J., Leworthy, D.P.: Metabolism of N,N'-diphenylurea by cytokinin-dependent tobacco callus: identification of the glucoside-Biochem. biophys. Res. Commun. 70: 1109-1114, 1976. Go to original source...
    4. Burch, L.R., Horgan R.: The purification of cytokinin oxidase from Zea mays kernels-Phytochemistry 28: 1313-1319, 1989. Go to original source...
    5. Capelle, S.C., Mok, D.W.C., Kirchner, S.C., Mok, M.C.: Effects of thidiazuron on cytokinin autonomy and the metabolism of N6-(Delta;2-isopentenyl)[8-14C]adenosine in callus tissues of Phaseolus lunatus L.-Plant Physiol. 73: 796-802, 1983. Go to original source...
    6. Chatfield, J.M., Armstrong, D.J.: Regulation of cytokinin oxidase in callus tissue of Phaseolus vulgaris L. cv. Great Northern-Plant Physiol. 80: 493-499, 1986. Go to original source...
    7. Dyson, W.H., Fox, J.E., McChesney, J.D.: Short term metabolism of urea and purine cytokinins.-Plant Physiol. 49: 506-513, 1972. Go to original source...
    8. Hansen, C.E., Kopperud, C., Heide, O.M.: Immunoaffinity chromatography for purification of cytokinins in plant extracts-Norw. J. agr. Sci. 3: 73-77, 1989. Go to original source...
    9. Hutchinson, M.J., Saxena, P.K.: Role of purine metabolism in thidiazuron-induced somatic embryogenesis of geranium (Pelargonium × hortorum) hypocotyl cultivars-Physiol. Plant. 98: 517-522, 1996. Go to original source...
    10. Kaminek, M., Armstrong, D.J.: Genotypic variation in cytokinin oxidase from Phaseolus callus cultures-Plant Physiol. 93: 1530-1538, 1990. Go to original source...
    11. Kaminek, M., Motyka, V., Vaòková, R.: Regulation of cytokinin content in plant cells-Physiol. Plant. 101: 689-700, 1997. Go to original source...
    12. Laloue, M., Fox, J.E.: Characterisation of an imine intermediate in the degradation of isopentenylated cytokinins by a cytokinin oxidase from wheat-In: Bopp, M., Knoop, B., Rademacher, W. (ed.): Abstracts of the 12th International Conference on Plant Growth Substances. P. 23. International Plant Growth Association, Heidelberg 1985.
    13. Laloue, M., Fox, J.E.: Cytokinin oxidase from wheat. Partial purification and general properties-Plant Physiol. 90: 899-906, 1989. Go to original source...
    14. Meins, F.: Habituation of cultured cells for cytokinins-In: Mok, D.W.S., Mok, M.C. (ed.): Cytokinins, Chemistry, Activity and Function. Pp. 269-287. CRC Press, Boca Raton 1994. Go to original source...
    15. Miller, C.: Kinetin and related compounds in plant growth-Annu. Rev. Plant Physiol. 12: 395-408, 1961. Go to original source...
    16. Mok, M.C., Mok, D.W.S.: The metabolism of [14C]thidiazuron in callus tissues of Phaseolus lunatus L.-Physiol. Plant. 65: 427-432, 1985. Go to original source...
    17. Mok, M.C., Kim, S.-G., Armstrong, D.J., Mok, D.W.S.: Induction of cytokinin autonomy by N,N'-diphenylurea in tissue cultures of Phaseolus lunatus L.-Proc. nat. Acad. Sci. USA 76: 3880-3884, 1979. Go to original source...
    18. Mok, M.C., Mok, D.W.S., Turner, J.E., Mujer, C.V.: Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems.-HortScience 22(Suppl.): 1194-1197, 1987. Go to original source...
    19. Motyka, V., Kamínek, M.: Characterisation of cytokinin oxidase from tobacco and poplar callus cultures-In: Kamínek, M., Mok, D.W.S., Za¾ímalová, E. (ed.): Physiology and Biochemistry of Cytokinins in Plants. Pp 33-39. SBP Academic Publishing, The Hague 1992.
    20. Parker, C.W., Entsch, B., Letham, D.S.: Inhibitors of two enzymes which metabolize cytokinins-Phytochemistry 25: 303-310, 1986. Go to original source...
    21. Schwartzenberg, K., von: [Der Cytokiningehalt in Nadeln unterschiedlich Stark von "neuartigen waldschäden betzoffener" Fichten (Picea abies [L.] Karst.), bestimmt mittels einer immunoenzymatischen Methode-ELISA.]-PhD Thesis. University of Bonn, Bonn 1989. [In German.]
    22. Shudo, K.: Chemistry of phenylurea cytokinins-In: Mok, D.W.S., Mok, M.C. (ed.): Cytokinins, Chemistry, Activity and Function. Pp. 35-42. CRC Press, Boca Raton 1994. Go to original source...
    23. Thomas, J.C., Katterman, F.R.: Cytokinin activity induced by thidiazuron.-Plant Physiol. 81: 681-683, 1986. Go to original source...

    Return to the content