Biologia plantarum 44:455-457, 2001 | DOI: 10.1023/A:1012448306010
Low Genetic Polymorphism in Natural Populations of Crotalaria Longipes
- 1 M.S. Swaminathan Research Foundation, Chennai-, India
- 2 National Research Center for Oilpalm, Trivandrum-, India
Natural genetic variation present in two populations of the critically endangered legume C. longipes was revealed by random amplified polymorphic DNA (RAPD) analysis. Out of the 30 primers used to test the intra-specific genetic polymorphism (between individuals from isolated populations) only 21 gave amplification. Eight primers produced monomorphic bands and 13 primers produced polymorphism. But the range of percent polymorphism was only 0 to 33 %. There was close similarity between individuals within and between populations. Cluster analysis based on Nei's indices did not reveal any population differentiation and individuals of both populations clustered with each other. These results point to a very low genetic polymorphism in C. longipes populations.
Keywords: endangered legume; intra-specific genetic polymorphism
Subjects: Crotalaria longipes; intra-specific genetic polymorphism; polymorphism, genetic, intra-specific; random amplified polymorphic DNA
Published: September 1, 2001 Show citation
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