biologia plantarum

Biologia plantarum 44:463-466, 2001 | DOI: 10.1023/A:1012452406918

Salt Tolerance of In Vitro Established Salt-Tolerant Rice Plants during Further Growth in Soil

Y. Miki¹, M. Katoh¹, S. Hisajima¹

¹ Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki, Japan

In vitro salt tolerant rice plants established by step up treatment with 0.5, 1.0, 1.5 and 2.0 % NaCl at 3-week intervals were examined to determine whether they could grow in potted paddy soil containing 0, 0.55 or 0.75 % NaCl till harvesting. All the control plants were necrotic by the 4^th week in the culture. At the 10^th week of culture, 100 % of the salt-tolerant plants subjected to 0 or 0.55 % NaCl survived, and 78 % of the plants at 0.75 % NaCl. The Na⁺ and Cl^- contents in the leaves of salt-tolerant plants grown at 0.55 and 0.75 % NaCl were about 4 times of those without NaCl. The ion contents in non-tolerant plants and seedling plants were 10 to 12 times of those in 0 % NaCl treatment. One of the hypotheses to explain the present data is that the in vitro step up salt selection induces the capability to maintain no lethal concentration of NaCl in the leaves.

Keywords: adaptation; cellular NaCl concentration; Oryza saliva L.; step up salt treatment

Subjects: NaCl concentration, cellular; Oryza sativa; rice; salt tolerance, in vitro established, ex vitro growth; salt tolerance through step up NaCl treatment, ex vitro growth

Published: September 1, 2001  Show citation