biologia plantarum

Biologia plantarum 44:489-495, 2001 | DOI: 10.1023/A:1013761814407

Somaclonal Variation in Rice after Two Successive Cycles of Mature Embryo Derived Callus Culture in the Presence of NaCl

S. Lutts¹, J.-M. Kinet¹, J. Bouharmont¹

¹ Laboratoire de Cytogénétique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium

Two successive cycles of mature embryo-derived callus culture separated by one cycle of sexual reproduction of R₀ regenerated plants were performed using two rice (Oryza sativa L.) cultivars in order to gain information upon the nature of somaclonal variation in this species. Plants regenerated after one cycle of tissue culture exhibited higher variability and lower performances than those of initial cultivar. A second cycle performed using R₁ embryos as explants showed that the cellular component of salt resistance in terms of growth and regenerating abilities selected during the first cycle could be transmitted to the progenies. The extent and the nature of somaclonal variation depended on the identity of R₀ mother plant and culture conditions, somaclonal variation being strongly reduced in some families obtained from salt-treated calli.

Keywords: genetic variation; in vitro selection; Oryza sativa; salinity; tissue culture

Subjects: auxins; cytokinins; genetic variation, embryo derived callus culture, NaCl; in vitro culture, salt stress; in vitro culture, somaclonal variation, salt stress; Oryza sativa; salt stress, embryo derived callus culture

Published: December 1, 2001  Show citation