biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:541-545, 2001 | DOI: 10.1023/A:1013738603020

Alleviation of Changes in Protein Metabolism in NaCl-Stressed Wheat Seedlings by Thiamine

F. El-Shintinawy1, M.N. El-Shourbagy1
1 Botany Department, Faculty of Science, Tanta University, Tanta, Egypt

NaCl-stress induced a pronounced suppression in growth of wheat seedlings. The most abundant amino acids (cysteine, arginine, methionine) constituting about 55 % of total free amino acid content in control wheat were reduced in 100 mM NaCl-treated plants. However, valine, isoleucine, aspartic acid and proline accumulated in response to NaCl stress and NaCl-treated wheat seedlings showed 1.6 fold increase in total free amino acids compared to the control. Addition of 2 [micro ]M thiamine alleviated the effects of NaCl on the amino acid composition and the amount of total free amino acids decreased to that in the control. Content of 26 kDa protein increased in NaCl-treated plants, stimulation was more pronounced in roots than in shoots. In contrast, the contents of 13 and 20 kDa proteins decreased. After addition of thiamine, the 24 kDa protein, which disappeared with NaCl treatment, has been initiated again. Moreover, thiamine treatment stimulated the accumulation of the 20 kDa protein.

Keywords: amino acids; HPLC; protein patterns; salinity; Triticum aestivum
Subjects: amino acids, salt stress, alleviation by thiamine; protein metabolism, alleviation of salt stress; salt stress, protein metabolism; thiamine, alleviation of salt stress; Triticum aestivum; wheat

Published: December 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
El-Shintinawy, F., & El-Shourbagy, M.N. (2001). Alleviation of Changes in Protein Metabolism in NaCl-Stressed Wheat Seedlings by Thiamine. Biologia plantarum, 44(4), 541-545. doi: 10.1023/A:1013738603020
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bouchereau, A., Aziz, A., Larher, F., Matin-Tanguy, J.: Polyamines and environmental challenges: recent development.-Plant Sci. 140: 103-125, 1999. Go to original source...
    2. Bradford, M.: A rapid and sensitive method for the quantitation of protein utilizing the principal of protein dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. Chen, C.C.S., Plant, A.L.: Salt-induced protein synthesis in tomato roots: the role of ABA.-J. exp. Bot. 50: 677-687, 1999. Go to original source...
    4. Delauney, A., Verma, D.: Proline biosynthesis and osmoregulation in plants.-Plant J. 4: 215-223, 1993. Go to original source...
    5. Dell'Aquila, A., Spada, P.: Regulation of protein synthesis in germinating wheat embryos under polyethylene glycol and salt stress.-Seed Sci. Res. 2: 75-80, 1992. Go to original source...
    6. DeLong, J., Steffen, K.: Lipid peroxidation and α-tocopherol content in α-tocopherol supplemented thylakoid membranes during UV-B exposure.-Environ. exp. Bot. 39: 177-185, 1998. Go to original source...
    7. El-Shintinawy, F., El-Ansary, A.: Differential effect of Cd and Ni on amino acid metabolism in soybean seedings.-Biol. Plant. 43: 79-84, 2000. Go to original source...
    8. El-Shintinawy, F., El-Shourbagy, M.: Recovery of photosystem 2 and membrane lipid composition in triazine-treated soybean seedlings by vitamins.-Biol. Plant. 39: 633-636, 1997. Go to original source...
    9. el-Shourbagy, M., Abdulla, O., Ahmed, F.: Effects of constant and gradual exposure to sodium chloride stress on DNA, RNA, protein and certain protein-amino acids in two varieties of barley.-Phyton 20: 215-225, 1980.
    10. Godoy, J., Pardo, J., Pintor-Toro, J.: A tomato cDNA inducible by salt stress and abscisic acid: nucleotide sequence and expression pattern.-Plant mol. Biol. 15: 695-705, 1990. Go to original source...
    11. Guerrier, G.: Proline accumulation in salt-treated tomato: different proline precursors in Lycopersicon esculentum and Lycopersicon pennellii.-J. Plant Nutr. 21: 505-513, 1998. Go to original source...
    12. Gulick, P., Dvorak, J.: Gene induction and repression by salt treatment in roots of the salinity-sensitive Chinese spring wheat and the salinity-tolerant Chinese spring wheat × Elytrigia elongata amphiploid.-Proc. nat. Acad. Sci. USA 84: 99-103, 1987. Go to original source...
    13. Hernandez, J., Corpas, F., Gomez, M., delRio, L, Sevilla, F.: Salt-induced oxidative stress mediated by activated oxygen species in pea leaf mitochondraia.-Physiol. Plant. 89: 103-110, 1993. Go to original source...
    14. Hernandez, J., Olmos, E., Corpas, F., Sevilla, F., delRio, L.: Salt-induced oxidative stress in chloroplasts in pea plants.-Plant Sci. 105: 151-167, 1995. Go to original source...
    15. Hurkman, W., Tanaka, C., Fornari, C.: A comparison of the effects of salt on polypeptides and translatable mRNAs in the roots of a salt-tolerant and a salt-sensitive cultivar of barley.-Plant Physiol. 90: 1444-1456, 1989. Go to original source...
    16. Izzo, R., Navari-Izzo, F., Quartacci, M.: Growth and mineral absorption in maize seedlings as affected by increasing NaCl concentrations.-J. Plant Nutr. 14: 687-699, 1991. Go to original source...
    17. Kuznetsov, V., Shevyakova, I.: Stress responses of tobacco cells to high temperature and salinity. Proline accumulation and phosphorylation of polypeptides.-Physiol. Plant. 100: 320-326, 1997. Go to original source...
    18. La Rosa, P. Chen, Z., Nepson, D., Singh, N., Hasegawa, P., Bressan, R.: Osmotin gene expression is post-transcriptionally regulated.-Plant Physiol. 100: 409-415, 1992.
    19. Laemmli, U.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-686, 1970. Go to original source...
    20. Lopez, F., Vansuyt, G., Foureroy, P. Casse-Delbart, F.: Accumulation of a 22-kDa protein and its mRNA in the leaves of Raphanus sativus in response to salt stress or water deficit-Physiol. Plant. 91: 605-614, 1994.
    21. Mansour, M., Salama, K.: Comparative responses to salinity in wheat genotypes differing in salt tolerance. 1 - Seedling growth and mineral relations.-Egypt. J. Physiol. 20: 1-15, 1996.
    22. Ramagopal, S.: Salinity stress induced tissue-specific proteins in barely seedlings.-Plant Physiol. 84: 324-331, 1987. Go to original source...
    23. Rennenberg, H.: Glutathione metabolism and possible biological roles in higher plants.-Phytochemistry 21: 2771-2781, 1982. Go to original source...
    24. Scarponi, L., Nemat Alla, M., Martinetti, L.: Consequences on nitrogen metabolism in soybean (Glycine max L.) as a result of imazethapyr action acetohydroxy acid synthase.-J. Agr. Food Chem. 43: 809-814, 1995. Go to original source...
    25. Velitchkova, M., Fedina, I.: Response of photosynthesis of Pisum sativum to salt stress as affected by methyl jasmonate.-Photosynthetica 35: 89-97, 1998. Go to original source...
    26. Weibull, J., Ronquist, F., Brishammar, S.: Free amino acid composition of leaf exudates and phloem sap.-Plant Physiol. 92: 222-226, 1990. Go to original source...
    27. Weigel, P., Weretilnyk, E., Hanson, A.: Betaine aldehyde oxidation by spinach chloroplasts.-Plant Physiol. 82: 753-759, 1986. Go to original source...

    Return to the content