biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:579-585, 2001 | DOI: 10.1023/A:1013798821676

Hop Latent Viroid (HLVd)-Caused Pathogenesis: Effects of HLVd Infection on Lupulin Composition of Meristem Culture-Derived Humulus lupulus

J. Patzak1, J. Matoušek2, K. Krofta1, P. Svoboda1
1 Hop Research Institute Co., Žatec, Czech Republic
2 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic

Season-dependent fluctuation of hop latent viroid in meristem tips enabled selection of viroid-free cultures from Osvald's clone 31, 72, 114, and cv. Premiant. These mericlones were used to evaluate effect of viroid infection on the composition of hop resins and essential oils in the first production year. Healthy plants were compared with naturally re-infected individuals under field conditions. On average, viroid infection decreased content of α-bitter acids by 40 %. The content of β-bitter acids, as well as the ratios of humulone/cohumulone and lupulone/colupulone was not influenced by viroid infection. The content of all monoterpenes was for 29, 37.4 and 41.6 % higher for myrcene, α- and β-pinene, respectively, in infected plants compared with the healthy controls. The contents of sesquiterpenes like β-caryophyllen α-humulene, α-copaene, γ-muurolene, β-bisabolene, γ-cadinene, and δ-cadinene decreased by 13.7, 13, 14, 18.5, 29, 21.7 and 18.5 %, respectively, due to viroid infection. The possible influence of some oxidative-reduction processes activated by viroid-caused pathogenesis was assumed to be involved in the accumulation of terpenes alcohols like geraniol and methylgeranate, and in the reduction of the contents of the majority of ketones detected in the spectra of essential oils.

Keywords: hop; in vitro mericlones; RT PCR diagnosis; viroid elimination
Subjects: hop latent viroid infection, lupulin, meristem culture; Humulus lupulus; in vitro culture, infection, defense mechanism; lupulin; mericlones in vitro , hop latent viroid infection

Published: December 1, 2001  Show citation

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Patzak, J., Matoušek, J., Krofta, K., & Svoboda, P. (2001). Hop Latent Viroid (HLVd)-Caused Pathogenesis: Effects of HLVd Infection on Lupulin Composition of Meristem Culture-Derived Humulus lupulus. Biologia plantarum, 44(4), 579-585. doi: 10.1023/A:1013798821676
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