biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 44:607-609, 2001 | DOI: 10.1023/A:1013715425310

Effects of NaCl and Proline on Polyphenol Oxidase Activity in Bean Seedlings

Y. Demir1, İ. Kocaçalişkan2
1 K.K. Eğitim Fakültesi, Biyoloji Bölümü, Atatürk Üniversitesi, Erzurum, Türkey
2 Fen-Edebiyat Fakültesi Biyoloji Bölümü, Dumlupinar Üniversitesi, Kütahya, Türkey

In this work, the effects of NaCl (0, 50, 100, and 150 mM), proline (0, 5 and 10 mM) and NaCl + proline in combinations on activity of polyphenol oxidase (PPO; E.C. 1.10.3.1) and soluble protein content have been investigated in the root, stem and leaf tissues of bean (Phaseolus vulgaris L.) seedlings grown in embryo culture. PPO activities were higher in all the tissues treated with NaCl, proline and NaCl + proline combinations those that of the control tissues. The protein content was very high in tissues exposed to proline and NaCl + proline combination, but NaCl alone decreased protein contents in root and leaf tissues. The results suggest that proline may play a role as an enzyme-stabilizing agent in salt stress.

Keywords: embryo culture; Phaseolus vulgaris; salt stress
Subjects: bean; embryo culture, polyphenol oxidase, salt stress; in vitro culture, salt stress; Phaseolus vulgaris; polyphenol oxidase, salt stress; proline, polyphenol oxidase, salt stress; salt stress, polyphenol oxidase, embryo culture; salt stress, proline, polyphenol oxidase

Published: December 1, 2001  Show citation

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Demir, Y., & İ., K. (2001). Effects of NaCl and Proline on Polyphenol Oxidase Activity in Bean Seedlings. Biologia plantarum, 44(4), 607-609. doi: 10.1023/A:1013715425310
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