biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:611-613, 2001 | DOI: 10.1023/A:1013767426219

Stimulation of Shoot Regeneration on Linum Hypocotyl Segments by Thidiazuron and Its Response to Light and Calcium

P. Jain1, A. Rashid1
1 Department of Botany, University of Delhi, Delhi-, India

The basal segment from hypocotyl of Linum usitatissimum L. seedling readily regenerates, to produce a large number of shoots, in a short period of 5 [ndash ] 7 d. This response was stimulated by a low concentration (0.1 [micro ]M) of thidiazuron (TDZ). TDZ was also effective in inducing regeneration in dark. A drastic reduction in regeneration response on hormone-free as well as TDZ-supplemented medium was found after inclusion of an inhibitor of calcium-uptake, lanthanum (La3+). An essentiality of calcium in the regeneration was also evidenced by an increased response with increasing concentration of calcium. At Ca2+ concentration insufficient for regeneration, inclusion of TDZ resulted in shoot formation.

Keywords: calcium-inhibitor; calmodulin-inhibitor; chlorpromazine; flax; lanthanum; trifluoperazine
Subjects: calcium inhibitor, shoot regeneration, thidiazuron; calmodulin, shoot regeneration; chlorpromazine, shoot regeneration; flax; hypocotyl, shoot regeneration; in vitro culture, micropropagation, regeneration; lanthanum, shoot regeneration, thidiazuron; Linum usitatissimum; thidiazuron, shoot regeneration

Published: December 1, 2001  Show citation

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Jain, P., & Rashid, A. (2001). Stimulation of Shoot Regeneration on Linum Hypocotyl Segments by Thidiazuron and Its Response to Light and Calcium. Biologia plantarum, 44(4), 611-613. doi: 10.1023/A:1013767426219
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