biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 44:615-618, 2001 | DOI: 10.1023/A:1013771510289

Effect of Photoperiod and Chlorogenic Acid on Morphogenesis in Leaf Discs of Streptocarpus Nobilis

E.I.S. Floh1, W. Handro1
1 Plant Cell Biology Laboratory, Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, Brazil

Leaf discs from vegetative plants greatly increase their phenolic content when cultivated in vitro. Under long days the values remained constant, and were higher when compared with short days cultures. Under short days total phenolics decreased after 10 d, corresponding to the induction and expression of in vitro flowering. The effect of photoperiod and chlorogenic acid (0.01 mM) on leaf discs cultured from induced and non-induced plants, were analyzed regarding the neo-formation of roots, as well as vegetative and flower buds. Chlorogenic acid enhances the regeneration of roots in all treatments tested, with the highest stimulation on induced leaf discs cultivated in short days. The flowering was not affected by chlorogenic acid, but an inhibitory effect was observed on the neo-formation of vegetative buds in non-induced explants maintained in short days. Vegetative buds were reduced by 50% in flower-induced leaf discs cultivated under short days.

Keywords: flowering; phenols; rooting
Subjects: chlorogenic acid, photoperiod, morphogenesis; flowering, in vitro, phenols, rooting; in vitro culture, root formation; phenolics, rooting, chlorogenic acid; photoperiod, morphogenesis; Streptocarpus nobilis

Published: December 1, 2001  Show citation

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Floh, E.I.S., & Handro, W. (2001). Effect of Photoperiod and Chlorogenic Acid on Morphogenesis in Leaf Discs of Streptocarpus Nobilis. Biologia plantarum, 44(4), 615-618. doi: 10.1023/A:1013771510289
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    References

    1. Barz, W., Hoesel, W.: Metabolism and degradation of phenolic compounds in plants.-In: Swain, T., Harborne, J.B., Sumere, C.F. (ed.): Biochemistry of Plant Phenolics. Volume 12. Pp. 339-370. Plenum Press, New York-London 1979. Go to original source...
    2. Berthon, J.Y., Tahar, S.B., Gaspar, T., Boyer, N.: Rooting phases of shoots of Sequoiadendron giganteum in vitro and their requirements.-Plant Physiol. Biochem. 28: 631-638, 1990.
    3. Druart, P., Kevers, C., Boxus, P., Gaspar, T.: In vitro promotion of root formation by apple shoots through darkness effect on endogenous phenols and peroxidases.-Z. Pflanzenphysiol. 108: 429-436, 1982. Go to original source...
    4. Folin, O., Denis, W.: On phosphotungstic-phosphomolybdic compounds as color reagents.-J. biol. Chem. 12: 239-243, 1912. Go to original source...
    5. Gaspar, T.: Catabolisme auxinique et effecteurs auxines-oxydasiques. Étude comparée chez Lens culinaris et Salvia splendens.-Bull. Soc. roy. Sci. Liège 34: 391-537, 1965.
    6. Gaspar, T., Penel, C., Hagége, D.C., Greppin, H.: Peroxidases in plant growth, differentiation and development process.-In: Lobarzewski, J., Greppin, H., Penel, C., Gaspar, T. (ed.): Biochemical, Molecular, and Physiological Aspects of Plant Peroxidases. Pp. 249-280. University of Geneva, Geneva 1991.
    7. Gaspar T., Kevers, C, Hausman, J.F., Ripetti, V.: Peroxidase activity and endogenous free auxin during adventitious root formation.-In: Lumsden, P.J., Nicholas, J.R., Davies, W.J. (ed.): Physiology, Growth and Development of Plants in Culture. Pp. 289-298. Kluwer Academic Publishers, Amsterdam 1994. Go to original source...
    8. Gautheret, R.J.: La Culture des Tissues Végétaux.-Masson et Cie, Paris 1959.
    9. George, E.F.: Plant Propagation by Tissue Culture. 2nd Edition.-Eversley, Exegetics 1993.
    10. George, E.F., Sherrington, P.D.: Plant Propagation by Tissue Culture.-Eversley, Exegetics 1984.
    11. Hackett, W.P.: The influence of auxin, catechol and methanolic tissue extracts on root initiation in asseptically cultured shoot apices of the juvenile and adult forms of Hedera helix.-J. amer. Soc. hort. Sci. 95: 398-402, 1970.
    12. Hammerschlag, F.: Factors influencing in vitro multiplication and rooting of the plum roostock myrobalan (Prunus cerasifera Ehrh.).-J. amer. Soc. hort. Sci. 107: 44-47, 1982.
    13. Handro, W.: On the flower initiation in Streptocarpus nobilis C.B. Clarke (Gesneriaceae).-Bol. Bot. Univ. São Paulo 4: 31-39, 1976.
    14. Kefeli, V.I., Kadyrov, C.S.: Natural growth inhibitors, their chemical and physiological properties.-Annu. Rev. Plant Physiol. 22: 185-196, 1971. Go to original source...
    15. Margara, J.: Effets d'auxines et d'antiauxines sur la néoformations de bourgeons chez le chou-fleur (Brassica olearacea L. var. botrytis).-Compt. rend. Acad. Sci. Paris 284D: 1883-1885, 1977.
    16. Margara, J., Leydecker, M.T.: Différents types d'organogenèse observés chez le Colza, Brassica napus L. var. oleifera Metzg.-Compt. rend. Acad. Sci. Paris 287D: 17-20, 1978.
    17. Monteuuis, O., Bom, M.C., Berthon, J.Y.: Micropropagation aspects of Sequoiadendron giganteum juvenile and mature clones.-Acta Hort. 212: 489-497, 1987. Go to original source...
    18. Nitsch, C.: Induction in vitro de la floraison chez une plante de jours courts: Plumbago indica L.-Ann. Sci. Nat., bot. Ser. XII 9: 1-92, 1967.
    19. Nitsch, J.P., Nitsch, C.: Néoformation des fleurs in vitro chez une espèce de jours courts: Plumbago indica L.-Ann. Physiol. vég. 7: 1251-1256, 1965.
    20. Paulet, P.: Étude de la néoformation in vitro de bourgeons végétatifs et floraux.-Rev. gen. bot. 72: 697-792, 1965.
    21. Paulet, P., Nitsch, J.P.: La néoformation de fleurs sur cultures in vitro de Cichorium intybus L. Étude physiologique.-Ann. Physiol. vég. 6: 335-345, 1964.
    22. Rawal, S.K., Mehta, A.R.: Changes in enzymes activity and isoperoxidases in haploid tobacco callus during organogenesis.-Plant Sci. Lett. 24: 67-77, 1982. Go to original source...
    23. Sargent, J.A., Skoog, F.: Effects of indoleacetic acid and kinetin on scopoletin-scopolin levels in relation to growth of tobacco tissues in vitro.-Plant Physiol. 35: 934-941, 1960. Go to original source...
    24. Simmonds, J.A.: In vitro photoinduction of leaf tissue of Streptocarpus nobilis. The influence of culture medium components on vegetative and reproductive development.-Can. J. Bot. 60: 1461-1468, 1982. Go to original source...
    25. Simmonds, J.A.: In vitro photoinduction of leaf tissue of Streptocarpus nobilis.-Biol. Plant. 27: 318-324, 1985. Go to original source...
    26. Skoog, F., Montaldi, E.: Auxin-kinetin interaction regulating the scopoletin and scopolin levels in tobacco tissue cultures.-Proc. nat. Acad. Sci. USA 47: 36-49, 1961. Go to original source...
    27. Tryon, K.: Scopoletin in differentiating and non-differentiating cultured tobacco tissue.-Science 123: 590, 1956. Go to original source...
    28. Wescott, R.T., Henshaw, G.G.: Phenolic synthesis and phenylalanine ammonia-lyase activity in suspension cultures of Acer pseudoplatanus L.-Planta 131: 67-73, 1976. Go to original source...

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