biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 44:631-634, 2001 | DOI: 10.1023/A:1013779712106

Response of Zea mays to the Inoculation with Azospirillum on Nitrogen Metabolism under Greenhouse Conditions

C.M. Ribaudo1, D.P. Rondanini1, J.A. Curá1, A.A. Fraschina1
1 Cátedra de Bioquímica, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina

The maize (Zea mays L.) plants inoculated by N2-fixing bacterium Azospirillum showed increased activity of glutamate dehydrogenase (GDH) and glutamine synthetase (GS) in root cells free extracts over uninoculated control plants. Maximum differences in NADH-GDH activity were observed during the second and third weeks after sowing. The specific activity of GS showed a greater increase at the end of the assay. The percentage of nitrogen in leaves, root and foliage length, total fresh mass and nitrogenase activity were higher in inoculated plants than in the control ones.

Keywords: glutamate dehydrogenase; glutamine synthetase; maize; nitrogen content
Subjects: Azospirillum inoculation; glutamate dehydrogenase, Azospirillum inoculation; glutamine synthetase, Azospirillum inoculation; maize; Zea mays

Published: December 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ribaudo, C.M., Rondanini, D.P., Curá, J.A., & Fraschina, A.A. (2001). Response of Zea mays to the Inoculation with Azospirillum on Nitrogen Metabolism under Greenhouse Conditions. Biologia plantarum44(4), 631-634. doi: 10.1023/A:1013779712106
Download citation

References

  1. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
  2. Bulow, F.W.J. von, Döbereiner, J.: Potential for nitrogen fixation in maize genotypes in Brazil.-Proc. nat. Acad. Sci. USA 72: 2389-2393, 1975. Go to original source...
  3. Fallik, E., Okon, Y., Fisher, M.: The effect of Azospirillum brasilense inoculation on metabolic enzymes activity in maize roots seedlings.-Symbiosis 6: 1-8, 1988.
  4. Döbereiner, J., Baldani, V.L.D., Baldani, J.I. (ed.): Como isolar e identificar bactérias diazotróficas de plantas nãoleguminosas. [How to isolate and identify diazotroph bacteria on non-leguminous plants.]-R.J. EMBRAPA-CNPAB. Brasilia: EMBRAPA-SPI: Itaguaí 1995. [In Span.]
  5. Garg, S., Srivastava, H.S.: Seedling age and cytokininn effects on glutamate dehydrogenase activity and nitrogen assimilation in maize leaves.-Biol. Plant. 34: 153-157, 1992. Go to original source...
  6. Hardy, R.W.F., Holste, R.D., Jackson, E.K., Burns, R.C.: The acetylene ethylene assay for N2-fixation: laboratory and field evolution.-Plant Physiol. 43: 1185-1207, 1968. Go to original source...
  7. Hoagland, D.R., Arnon, D.I.: The water culture for growing plants without soil.-Circ. Calif. Agr. Exp. Sta. 347: 461-462, 1938.
  8. Kapulnik, Y., Okon, Y., Henis, Y.: Changes in root morphology of wheat caused by Azospirillum inoculation.-Can. J. Microbiol. 31: 881-887, 1985. Go to original source...
  9. Lang, C.A.: Simple microdetermination of Kjeldahl nitrogen in biological material.-Anal. Chem. 30: 1692-1694, 1958. Go to original source...
  10. Loyola Vargas, M.V., Sánchez de Jimenez, E.: Differential role of glutamate dehydrogenase in nitrogen metabolism of maize tissues.-Plant Physiol. 76: 536-540, 1984. Go to original source...
  11. Magalhães, J.R., Huber, D.M., Tsai, C.Y.: Influence of the form of nitrogen on ammonium, amino acids and N-assimilating enzyme activity in maize genotypes.-J. Plant Nutr. 18: 747-763, 1995. Go to original source...
  12. Michiels, K., Vanderleyden, J., Van Gool, A.: Azospirillum-plant root associations: a review.-Biol. Fertil. Soils 8: 356-368, 1989. Go to original source...
  13. Oaks, A., Stulen, I., Jones, K., Winspear, M.J., Misra, S., Boesel, I.: Enzymes of nitrogen assimilation in maize roots.-Planta 148: 477-484, 1980. Go to original source...
  14. Ribaudo, C.M., Rondanini, D.P., Paccusse, A.N., Curá, J.A., Fraschina, A.A.: Azospirillum-maize association: effects on dry matter yield and nitrate reductase activity.-Agr. trop. subtrop. 31: 61-70, 1998.
  15. Tien, T.M., Gaskins, M.H., Hubbel, D.H.: Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.).-Appl. environ. Microbiol. 37: 1016-1024, 1979. Go to original source...
  16. Yuan, H., Hu, C., Wu, H.: Ammonia assimilation and glutamine metabolism related to protein accumulation in developing rice grains.-Bot. Bull. Acad. sin. 31: 149-161, 1980.