biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:19-25, 2002 | DOI: 10.1023/A:1015134725621

Somatic Embryogenesis and Plant Regeneration in Pigeonpea

M.L. Mohan1, K.V. Krishnamurthy1
1 Plant Tissue Culture Division, National Chemical Laboratory, Pune, India

Somatic embryogenesis in pigeonpea [Cajanus cajan (L.) Millsp.] has been achieved using cotyledon segments of mature seeds as explants. A large number of globular somatic embryos were induced directly from cotyledons of genotypes T-15-15, GAUT-82-90 and GAUT-82-99 when cultured on EC6 basal medium supplemented with 2.22, 4.44, 13.32 or 22.2 μM N6-benzylaminopurine (BAP) and 0.45, 1.36, 2.27, 4.54 and 13.62 μM thidiazuron. Somatic embryos developed into cotyledonary stage when the globular embryos were transferred to Murashige and Skoog's (MS) basal medium containing 2.89 - 14.43 μM gibberellic acid. Maturation of somatic embryos was achieved on half strength MS medium with 0.38 μM abscisic acid. The mature somatic embryos were germinated on MS medium supplemented with 0.44 μM BAP and the plantlets were hardened and transferred to soil.

Keywords: abscisic acid; Cajanus cajan; cotyledon; EC6 medium; gibberellic acid; MS medium; N6-benzylaminopurine; thidiazuron
Subjects: abscisic acid; Cajanus cajan; cytokinins; gibberelins; in vitro culture, nutrient media modification; in vitro culture, somatic embryogenesis; pigeon pea, somatic embryogenesis; somatic embryogenesis; thidiazuron

Published: March 1, 2002  Show citation

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Mohan, M.L., & Krishnamurthy, K.V. (2002). Somatic Embryogenesis and Plant Regeneration in Pigeonpea. Biologia plantarum, 45(1), 19-25. doi: 10.1023/A:1015134725621
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