biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:27-32, 2002 | DOI: 10.1023/A:1015186709691

An Assessment of Genetic Integrity of Micropropagated Plants of Plumbago Zeylanica by RAPD Markers

G.R. Rout1, G. Das1
1 Plant Biotechnology Division, Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar, India

Clones of Plumbago zeylanica were micropropagated using nodal culture. The application of random amplified polymorphic DNA (RAPD) in assessing the genetic integrity of the micropropagated plants was evaluated by polymerase chain reaction. Twenty arbitrary decamers were used to amplify genomic DNA from in vitro and in vivo plant material to assess the genetic fidelity. All RAPD profiles from micro-propagated plants were monomorphic and similar to those of field grown mother plants. No polymorphism was detected within the micropropagated plants.

Keywords: genetic stability; in vitro; polymerase chain reaction
Subjects: genetic stability; in vitro culture, micropropagation; in vitro culture, nutrient media modification; in vitro culture, plant regeneration, organogenesis; Plumbago zeylanica; polymerase chain reaction; random amplified polymorphic DNA, RAPD

Published: March 1, 2002  Show citation

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Rout, G.R., & Das, G. (2002). An Assessment of Genetic Integrity of Micropropagated Plants of Plumbago Zeylanica by RAPD Markers. Biologia plantarum, 45(1), 27-32. doi: 10.1023/A:1015186709691
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