biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 45:45-49, 2002 | DOI: 10.1023/A:1015171701030

High Irradiance Induced Pigment Degradation and Loss of Photochemical Activity of Wheat Chloroplasts

R.K. Behera1, N.K. Choudhury1
1 School of Life Sciences, Sambalpur University, Jyoti Vihar, India

Loss of chlorophyll (Chl) and carotenoids (Car) of leaves and changes in Chl fluorescence emission and polarisation, malondialdehyde (MDA) accumulation, and 2,6-dichlorophenol indophenol (DCPIP) photoreduction in chloroplasts of wheat seedlings grown under different irradiance and subsequently exposed to high irradiance stress (HIS; 250 W m-2) were studied in mature and senescent primary wheat leaves. Faster rate of pigment loss was observed in leaves of moderate irradiance (MI; 15 W m-2) grown plants, compared to high irradiance (HI-1 and HI-2; 30 and 45 W m-2) ones when exposed to HIS. A relatively lower loss of Car in the plants grown in HI-1 and HI-2 exposed to HIS suggests HI adaptation of these seedlings. The slower rate of increase in the ratio of Chl fluorescence emission (F685/F735) also may suggest photoprotective strategy of HI grown seedlings. There was a positive correlation between MDA accumulation and Chl fluorescence polarisation. The DCPIP photoreduction activity in chloroplasts isolated from HI-1 and HI-2 grown plants exposed to HIS showed slower loss of electron transport activity compared to MI grown plants. These observations suggest that plants grown under higher irradiance have capacity to manage the excess quanta better than those grown under lower irradiance.

Keywords: carotenoids; chlorophyll; 2,6-dichlorophenol indophenol reduction; fluorescence; leaf age; malondialdehyde; photoinhibition; senescence
Subjects: carotenoids, chlorophyll, degradation; chlorophyll, carotenoids, degradation; fluorescence, chlorophyll, photoinhibition; high-irradiance stress; leaf age, photoinhibition, pigment degradation; malondialdehyde; ontogeny, leaf age; photochemical activities; photoinhibition; photon flux density; photosystem 2, photochemical activity; senescence, photoinhibition, pigment degradation; Triticum aestivum; wheat, high irradiance stress

Published: March 1, 2002  Show citation

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Behera, R.K., & Choudhury, N.K. (2002). High Irradiance Induced Pigment Degradation and Loss of Photochemical Activity of Wheat Chloroplasts. Biologia plantarum45(1), 45-49. doi: 10.1023/A:1015171701030
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References

  1. Barber, J., Nield, J., Morris, E.P., Zheleva, D., Hankamer, B.: The structure, function and dynamics if photosystem two.-Physiol. Plant. 100: 817-827, 1997. Go to original source...
  2. Behera, R.K., Choudhury, N.K.: Photosynthetic characteristics of chloroplasts of primary wheat leaves grown under different irradiance.-Photosynthetica 39: 11-15, 2001. Go to original source...
  3. Choudhury, N.K., Choe, H.T.: Photoprotective effect of kinetin on pigment content and photochemical activities of wheat chloroplasts aging in vitro.-Biol. Plant. 38: 61-69, 1996. Go to original source...
  4. Choudhury, N.K., Choe, H.T., Huffaker, R.C.: Ascorbate induced zeaxanthin formation in wheat leaves and photoprotection of pigment and photochemical activities during aging of chloroplasts in light.-J. Plant Physiol. 141: 551-556, 1993. Go to original source...
  5. Choudhury, N.K., Muhammad, A., Huffaker, R.C.: Photochemical activities in wheat chloroplasts incubated under irradiation and possible protection by zeaxanthin.-Photosynthetica 30: 397-405, 1994.
  6. Du, Z., Bramlage, W.J.: Modified thiobarbituric acid assay for measuring lipid oxidation in sugar rich plant tissue extracts.-J. agr. Food Chem. 40: 1566-1570, 1992. Go to original source...
  7. Genty, B., Harbinson, J.: Regulation of light utilization for photosynthetic electron transport.-In: Baker, N.R. (ed.): Photosynthesis and the Environment. Pp. 69-99. Kluwer Academic Publishers, Dordrecht 1996.
  8. Giardi, M.T., Masojídek, J., Godde, D.: Effect of abiotic stresses on the turnover of the D1 reaction center II protein.-Physiol. Plant. 101: 635-642, 1997. Go to original source...
  9. Gitelson, A.A., Buschmann, C., Lichtenthaler, H.K.: Leaf chlorophyll fluorescence corrected for reabsorption by means of absorption and reflectance measurements.-J. Plant Physiol. 152: 282-294, 1998. Go to original source...
  10. Gounaries, K., Barber, J., Harwood, J.L.: The thylakoid membranes of higher plant chloroplasts.-Biochem. J. 237: 313-326, 1986. Go to original source...
  11. Harbinson, J.: The responses of thylakoid electron transport and light utilization efficiency to sink limitation of photosynthesis.-In: Baker, N.R., Boyer, J.R. (ed.): Photoinhibition of Photosynthesis. From Molecular Mechanism to the Field. Pp. 273-296. Bios Scientific, Oxford 1994.
  12. Horton, P., Ruban, A.V., Walters, R.G.: Regulation of light harvesting in green plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 655-684, 1996. Go to original source...
  13. Ivanov, A.G., Morgan, R.M., Gray, G.R., Velitchkova, M.Y., Hurner, N.P.A.: Temperature/light dependent development of selective resistance to photoinhibition of photosystem I.-FEBS Lett. 430: 288-292, 1998. Go to original source...
  14. Kyle, D.J.: The biochemical basis for photoinhibition of photosystem II.-In: Kyle, D.J., Osmond, C.B., Arntzen, C.J. (ed.): Photoinhibition. Pp. 197-226. Elsevier, Amsterdam 1987.
  15. Lichtenthaler, H. K.: Chlorophylls and carotenoids - pigments of photosynthetic membranes.-Methods Enzymol. 48: 350-382, 1987. Go to original source...
  16. Lichtenthaler, H.K.: Vegetation stress: an introduction to the stress concept in plants.-J. Plant Physiol. 148: 4-14, 1996. Go to original source...
  17. Lichtenthaler, H.K., Burgastahler, R., Buschmann, C., Meier, D., Prenzel, U., Schonthal, A.: Effect of high light stress on composition, function and structure of the photosynthetic apparatus.-In: Marcelle, R. (ed.): Stress Effect on Photosynthesis. Pp. 353-370. Dr. W. Junk Publ., The Hague 1982. Go to original source...
  18. Lichtenthaler, H.K., Buschmann, C., Doll, M., Fietz, H.J., Bach, T., Kozel, U., Meier, D., Rahmsdorf, U.: Photosynthetic activity, chloroplast structure and leaf characteristics of high light and low light plants and of sun and shade leaves.-Photosynth. Res. 2: 115-141, 1981. Go to original source...
  19. Lichtenthaler, H.K., Miehe, J.A.: Fluorescence imaging as a diagnostic tool for plant stress.-Trends Plant Sci. 2: 316-320, 1997. Go to original source...
  20. Long, S.P., Humphries, S.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633-662, 1994. Go to original source...
  21. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light.-Annu. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
  22. Schiefthaler, U., Russell, A.W., Bolhar-Nordenkampf, H.R., Critchley, C.: Photoregulation and photodamage in Schefflera arboricola leaves adapted to different light environments.-Aust. J. Plant Physiol. 26: 485-494, 1999. Go to original source...
  23. Siefermann-Harms, D.: The light harvesting and protective functions of carotenoids in photosynthetic membrane.-Physiol. Plant. 68: 561-568, 1987. Go to original source...
  24. Swain, N.K., Choudhury, N.K., Raval, M.K., Biswal, U.C.: Differential changes in fluorescence characteristics in photosystem II rich grana fraction during aging in light and dark.-Photosynthetica 24: 135-142, 1990.
  25. Thayer, S.S., Björkman, O: Leaf xanthophyll content and composition in sun and shade determined by HPLC.-Photosynth. Res. 23: 331-343, 1990. Go to original source...
  26. Young, A.J.: The protective role of carotenoids in higher plants.-Physiol. Plant. 83: 702-708, 1991. Go to original source...