biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:59-63, 2002 | DOI: 10.1023/A:1015175802847

Light-Dark Changes in Proline Content of Barley Leaves under Salt Stress

I.S. Fedina1, K. Georgieva1, I. Grigorova1
1 Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

Proline accumulation in leaves of barley (Hordeum vulgare L. cv. Alfa) seedlings treated with 150 mM NaCl was promoted in the light and suppressed in the dark. The light/dark changes of proline content was enhanced with each 12 h light/12 h dark cycle and the proline content increased steadily. Root and shoot concentrations of Na+ and Cl- in salt treated plants increased about 10 to 25 times as compared to the control. The content of these ions and the content of malondialdehyde were higher in the shoot of seedlings exposed to salt stress for 4 d in the light in comparison with the seedlings exposed to NaCl for 4 d in darkness. Light stimulated both ions and proline accumulation in the leaves and has no effect in the roots. Oxygen uptake was higher in the seedlings kept 4 d in the light which have higher endogenous free proline content. Chlorophyll fluorescence measurements showed that the photochemical activity of PS 2 slightly decreased as a result of salt stress and was not influenced by light regimes during plant growth.

Keywords: chlorophyll fluorescence; Hordeum vulgare; ions accumulation; malondialdehyde; NaCl; oxygen uptake
Subjects: barley, proline content; fluorescence, chlorophyll, light-dark changes in proline content; Hordeum vulgare; malondialdehyde; oxygen uptake, salt stress; photochemical activities; photosystem 2, photochemical activity; proline; salt stress, photosynthesis, photochemical activity, chlorophyll; salt stress, proline

Published: March 1, 2002  Show citation

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Fedina, I.S., Georgieva, K., & Grigorova, I. (2002). Light-Dark Changes in Proline Content of Barley Leaves under Salt Stress. Biologia plantarum, 45(1), 59-63. doi: 10.1023/A:1015175802847
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