biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:117-120, 2002 | DOI: 10.1023/A:1015117027863

Enhancement of Green Gram Nodulation and Growth by Bacillus Species

S.S. Sindhu1, S.K. Gupta1, S. Suneja1, K.R. Dadarwal1
1 Department of Microbiology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India

Rhizobacteria belonging to Bacillus sp. were isolated from the rhizosphere of green gram (Vigna radiata). Seed inoculation with the rhizobacteria showed stunting effect on root growth whereas four Bacillus strains caused stimulation of shoot growth at both 4 and 7 d of observations. Coinoculation of some Bacillus strains with effective Bradyrhizobium strain S24 resulted in enhanced nodulation and plant growth of green gram. The shoot dry mass (ratio to uninoculated control) varied from 1.32 to 6.33 at day 30 and from 1.28 to 3.55 at day 40 of plant growth. Nodule promoting effect after 40 d of plant growth was observed with majority of Bacillus strains except for MRS9 and MRS26. Maximum gains in nodulation, nitrogenase activity and plant growth were observed with Bacillus strains MRS12, MRS18, MRS22 and MRS27 after 40 d of plant growth, suggesting the usefulness of introduced rhizobacteria in improving crop productivity.

Keywords: Bradyrhizobium; coinoculation; nitrogenase activity; rhizobacteria; seedling emergence; Vigna radiata
Subjects: Bacillus species, enhancement of nodulation; Bradyrhizobium; dinitrogen fixation; green gram, nodulation; inoculation of plants by Bacillus species; nitrogenase, nitrogen fixation; nodulation, enhancement by Bacillus species; rhizobacteria; Vigna radiata

Published: March 1, 2002  Show citation

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Sindhu, S.S., Gupta, S.K., Suneja, S., & Dadarwal, K.R. (2002). Enhancement of Green Gram Nodulation and Growth by Bacillus Species. Biologia plantarum, 45(1), 117-120. doi: 10.1023/A:1015117027863
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