biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:173-182, 2002 | DOI: 10.1023/A:1015176118719

Morphology and Anatomy of Pisum Sativum Somatic Embryos

M. Griga1
1 AGRITEC Ltd., ©umperk, Czech Republic

The morphological and anatomical aspects of direct and indirect somatic embryogenesis in pea were described. Direct embryos were induced from shoot apical meristems of 3 to 5-d-old pea seedlings, embryogenic callus originated from immature pea zygotic embryos or shoot apices. Auxin (picloram, 2,4-dichlorophenoxyacetic acid) was necessary to induce somatic embryos. The developmental stages typical for pea zygotic embryos were detected. Globular and heartshaped somatic embryos were morphologically similar to their zygotic counterparts; in contrast, torpedo and cotyledonary somatic embryos displayed great morphological variation, which affected mainly cotyledons (size, shape, number). Based on anatomical sections, possible ways of somatic embryo formation and localization of initiation sites within primary explant tissue have been proposed. The multicellular origin of somatic embryos is supposed in both systems of pea somatic embryogenesis under investigation.

Keywords: auxins; direct and indirect somatic embryogenesis; grain legumes; pea regeneration in vitro
Subjects: auxins; cytokinin antagonists, bud break and outgrowth; in vitro culture, somatic embryogenesis; pea, reneneration in vitro; Pisum sativum; somatic embryogenesis

Published: June 1, 2002  Show citation

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Griga, M. (2002). Morphology and Anatomy of Pisum Sativum Somatic Embryos. Biologia plantarum, 45(2), 173-182. doi: 10.1023/A:1015176118719
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    References

    1. Bencheikh, M., Gallais, A.: Study of the variation in the somatic embryogenesis ability of some pea lines (Pisum sativum L. and Pisum arvense L.).-Euphytica 90: 551-256, 1996. Go to original source...
    2. Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.-Exp. Cell Res. 50: 151-158, 1968. Go to original source...
    3. Griga, M.: Direct somatic embryogenesis from shoot apical meristems of pea and thidiazuron induced high conversion rate of somatic embryos.-Biol. Plant. 41: 481-495, 1998. Go to original source...
    4. Griga, M.: Somatic embryogenesis in grain legumes.-In: Strnad, M., Peč, P. (ed.): Advances in Regulation of Plant Growth and Development. Pp. 233-249. Peres Publ., Prague 1999.
    5. Griga, M.: Morphological alterations in sterile mutant of Pisum sativum obtained via somatic embryogenesis.-Biol. Plant. 43: 161-165, 2000. Go to original source...
    6. Griga, M., Klenotičová, H.: Development of embryogenic suspension culture in pea.-In: Extended Abstracts COST 843 Quality Enhancement of Plant Production through Tissue Culture. Pp. 20-21. Blankenberge 2000.
    7. Griga, M., Létal, J.: Somaclonal variation study of pea (Pisum sativum L.)-In: Recent Advances in Plant Biotechnology. Pp. 130-135. Inst. Plant Genet., Nitra 1995.
    8. Griga, M., Poąvec, Z., Klenotičová, H., Horáček, J.: Protein and starch metabolism during development of pea somatic embryos.-In: Proc. 4th Eur. Conf. Grain Legumes. AEP Publ., Cracow 2001 (in press).
    9. Griga, M., Stejskal, J., Beber, K.: Analysis of tissue culture-derived variation in pea (Pisum sativum L.) - preliminary results.-Euphytica 85: 335-339, 1995. Go to original source...
    10. Hartweck, L.M., Lazzeri, P.A., Collins, G.B., Williams, E.G.: Auxin-orientation effects on somatic embryogenesis from immature soybean cotyledons.-In Vitro cell. develop. Biol. 24: 821-828, 1988. Go to original source...
    11. Hepher, A., Boulter, M.E., Harris, N., Nelson, R.S.: Development of a superficial meristem during somatic embryogenesis from immature cotyledons of soybean (Glycine max L.).-Ann. Bot. 62: 513-519, 1988. Go to original source...
    12. Kara, A.N.: Initiation and growth of leaf primordia in the apices of pea explants: An analysis of sensitivity to phytohormone treatments.-Fiziol. Rast. 42: 175-186, 1995.
    13. Kysely, W., Jacobsen, H.-J.: Somatic embryogenesis from pea embryos and shoot apices.-Plant Cell Tissue Organ Cult. 20: 7-14, 1990. Go to original source...
    14. Kysely, W., Myers, J.R., Lazzeri, P.A., Collins, G.B., Jacobsen, H.-J.: Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.).-Plant Cell Rep. 6: 305-308, 1987. Go to original source...
    15. Lazzeri, P.A., Hildebrand, D.F., Collins, D.B.: Soybean somatic embryogenesis: Effect of nutritional and chemical factors.-Plant Cell Tissue Organ Cult. 10: 209-220, 1987. Go to original source...
    16. Lehminger-Mertens, R., Jacobsen, H.-J.: Plant regeneration from pea protoplasts via somatic embryogenesis.-Plant Cell Rep. 8: 379-382, 1989. Go to original source...
    17. Liu, C.M., Johnson, S., Hedley, C.L., Wang, T.L.: The generation of a legume embryo: morphological and cellular defects in pea mutants.-In: Wang, T.L., Cuming, A. (ed.): Embryogenesis, the Generation of a Plant. Pp. 191-213. Bios Sci. Publ., Oxford 1996.
    18. Liu, C.M., Johnson, S., Wang, T.L.: cyd, a mutant of pea that alters embryo morphology is defective in cytokinesis.-Dev. Genet. 16: 321-331, 1995. Go to original source...
    19. Loiseau, J., Marche, C., Le Deunff, Y.: Effects of auxins, cytokinins, carbohydrates and amino acids on somatic embryogenesis induction from shoot apices of pea.-Plant Cell Tissue Organ Cult. 41: 267-275, 1995. Go to original source...
    20. Loiseau, J., Marche, C., Le Deunff, Y.: Variability of somatic embryogenic ability in the genus Pisum L.: effects of genotype, explant source and culture medium.-Agronomie 16: 299-308, 1996. Go to original source...
    21. Loiseau, J., Michaux-Ferriere, N., Le Deunff, Y.: Histology of somatic embryogenesis in pea.-Plant Physiol. Biochem. 36: 683-687, 1998. Go to original source...
    22. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 472-497, 1962. Go to original source...
    23. Nadolska-Orczyk, A., Milkowska, L., Orczyk, W.: Two ways of plant regeneration from immature cotyledons of pea.-Acta Soc. Bot. Pol. 63: 153-157, 1994. Go to original source...
    24. Ozcan, S., Barghchi, M., Firek, S., Draper, J.: Efficient adventitious shoot regeneration and somatic embryogenesis in pea.-Plant Cell Tissue Organ Cult. 34: 271-277, 1993. Go to original source...
    25. Stejskal, J., Griga, M.: Somatic embryogenesis and plant regeneration in Pisum sativum L.-Biol. Plant. 34: 15-22, 1992. Go to original source...
    26. Tétu, T., Sangwan, R.S., Sangwan-Noreel, B.S.: Direct somatic embryogenesis and organogenesis in cultured immature zygotic embryos of Pisum sativum L.-J. Plant Physiol. 137: 102-109, 1990. Go to original source...
    27. Van Doorne, L.E., Marshall, G., Kirkwood, R.C.: Somatic embryogenesis in pea (Pisum sativum L.) - effect of explant, genotype and culture conditions.-Ann. appl. Biol. 126: 169-179, 1995. Go to original source...
    28. Williams, E.G., Maheswaran, G.: Somatic embryogenesis: factors influencing coordinated behaviour of cells as an embryogenic group.-Ann. Bot. 57: 443-462, 1986. Go to original source...
    29. Yeung, E.C.: Structural and developmental patterns in somatic embryogenesis.-In: Thorpe, T.A. (ed.): In Vitro Embryogenesis in Plants. Pp. 205-247. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...

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