biologia plantarum

Biologia plantarum 45:183-188, 2002 | DOI: 10.1023/A:1015128202790

Anticytokinin Effect on Apical Dominance Release in in vitro Cultured Rosa Hybrida L.

V. Kapchina-Toteva¹, M. Somleva², H.J. Van Telgen³

¹ Department of Plant Physiology, Faculty of Biology, University of Sofia, Sofia, Bulgaria

² Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

³ Centre for Plant Tissue Culture Research, Lisse, The Netherlands

Effects of two cytokinin antagonists - 2-chloro-4-cyclobutyl-amino-6-ethylamino-1,3,5-triazine and N-(4-pyridyl)-O-(4-chlorophenyl)carbamate - on bud break and outgrowth in single nodes from two Rosa hybrida cultivars differing in their apical dominance were studied. The compounds were applied at three different concentrations separately or in combination with benzyladenine. Cytokinin antagonists reduced the number of the sprouted buds in both cultivars at different extent. Their effect was strongly dependent on both concentration applied and culture period duration. The replacement of anticytokinins with benzyladenine in the medium resulted in overcoming of the bud break suppression. Both compounds significantly inhibited bud outgrowth as well.

Keywords: cytokinins; cytokinin antagonists; rose; single nodes

Subjects: anticytokinin, apical dominance; 2-chloro-4-cyclobutyl-amino-6-ethylamino-1,3,5-triazine, cytokinin antagonists; cultivar differences; cytokinins; N-(4-pyridyl)-O-(4-chlorophenyl)carbamate, cytokinin antagonists; Rosa hybrida; rose cultivars, apical dominance

Published: June 1, 2002  Show citation