biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 45:197-204, 2002 | DOI: 10.1023/A:1015132303698

Artichoke Leaf Morphology and Surface Features in Different Micropropagation Stages

C.B. Brutti1, E.J. Rubio1, B.E. Llorente1, N.M. Apóstolo1
1 Laboratorio de Cultivo de Tejidos Vegetales, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Buenos Aires, Argentina

Artichoke (Cynara scolymus L.) leaf size and shape, glandular and covering trichomes, stomatal density, stomata shape, pore area and epicuticular waxes during micropropagation stages were studied by scanning electron microscopy (SEM) and morphometric analysis with the aim to improve the survival rate after transfer to greenhouse conditions. Leaves from in vitro shoots at the proliferation stage showed a spatular shape, ring-shaped stomata, a large number of glandular trichomes and juvenile covering hairs, but failed to show any epicuticular waxes. Leaves from in vitro plants at the root elongation stage showed a lanceolated elliptic shape with a serrated border, elliptical stomata, decreased pore area percentage, stomatal density, and mature covering trichomes. One week after transfer to ex vitro conditions, epicuticular waxes appeared on the leaf surface and stomata and pore area were smaller as compared to in vitro plants. Artichoke acclimatization may be improved by hormonal stimulation of root development, since useful morphological changes on leaves occurred during root elongation.

Keywords: acclimatization; Cynara scolymus; in vitro culture; leaf epidermis; stomata; trichomes
Subjects: artichoke, micropropagation; Cynara scolymus; in vitro culture, micropropagation; in vitro culture, plant regeneration, organogenesis; leaf morphology, surface features, micropropagation stages; stomata, micropropagation stages; trichomes

Published: June 1, 2002  Show citation

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Brutti, C.B., Rubio, E.J., Llorente, B.E., & Apóstolo, N.M. (2002). Artichoke Leaf Morphology and Surface Features in Different Micropropagation Stages. Biologia plantarum, 45(2), 197-204. doi: 10.1023/A:1015132303698
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