biologia plantarum

Biologia plantarum 45:255-260, 2002 | DOI: 10.1023/A:1015105025080

Changes in Thiol Content in Roots of Wheat Cultivars Exposed to Copper Stress

I. Tari¹, G. Szalai², Zs. Lôrincz¹, A. Bálint²

¹ Department of Plant Physiology, University of Szeged, Szeged, Hungary

² Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary

Wheat (Triticum aestivum L.) cultivars GK Tiszatáj, Yubileinaya, GK Öthalom and a landrace Kobomugi were grown for 18 d in hydroponic cultures containing 0 (control), 0.1, 1.0 or 10.0 µM Cu²⁺. On a dry mass basis, cvs. Tiszatáj and Kobomugi accumulated slightly more Cu²⁺ in the root tissues than did cvs. Yubileinaya and Öthalom, but their controls also contained higher amounts of Cu²⁺. As a result of perturbation in the plasma membrane functions the K⁺ content of roots was reduced at 10 µM Cu²⁺ in all cultivars, whereas the K⁺/Na⁺ ratio decreased significantly only in the roots of cv. Öthalom. In the sensitive cultivar, Öthalom, the dry mass of the roots decreased while the cysteine content, which is a limiting factor for glutathione synthesis, did not satisfactorily increase with increasing tissue Cu²⁺ content. This suggests that in cv. Öthalom the membrane damage of the root cells at 10 µM Cu²⁺ concentration may affect the sulphur availability or metabolism. Concentrations of glutathione and hydroxymethyl-glutathione, a tripeptide which may play a similar biochemical role to glutathione, were also lower in the sensitive cultivar. In the absence of glutathione the root tissues failed to cope with the oxidative stress caused by the excessive amount of Cu²⁺. A significant accumulation of iron in the roots of the sensitive cultivar at 10 µM Cu²⁺ supply enhanced the oxidative damage.

Keywords: glutathione; γ-glutamylcysteine; hydroxymethyl-glutathione; phytochelatins; iron accumulation

Subjects: copper stress, thiol in roots; cultivar differences; γ-glutamylcystein, copper stress, thiols in roots; glutathione, copper stress, thiol; heavy metals; hydroxymethylglutathione, copper stress, thiol in roots; iron accumulation, copper stress, thiol in roots; phytochelatins, copper stress, thiol in roots; thiol, copper stress; Triticum aestivum; wheat, copper stress, thiol in roots

Published: June 1, 2002  Show citation