biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:273-279, 2002 | DOI: 10.1023/A:1015161110058

BTH-Induced Accumulation of Extracellular Proteins and Blackspot Disease in Rose

Y. Suo1, D.W.M. Leung1
1 Department of Plant and Microbial Sciences, University of Canterbury, Christchurch 1, New Zealand

Treatment of rose shoots with 50 µM acibenzolar-S-methyl (BTH) resulted in increased protection against Diplocarpon rosae. This was accompanied by the induction and accumulation of a set of extracellular proteins as shown by SDS-PAGE and 2D-PAGE. Some of these proteins have been identified as PR-1, PR-2, PR-3 and PR-5 proteins by immunoblot analysis probed with tobacco antisera against PR-1c, PR-N, PR-Q and PR-S protein. Most of the extracellular proteins activated by BTH were also induced and found to accumulate in leaves upon infection with Diplocarpon rosae. However, their accumulation was much more pronounced in BTH-pretreated leaves than in water-pretreated leaves upon a challenge inoculation with D. rosae, particularly, the 15 kD PR-1, 36 and 37 kD PR-2 proteins. They may be more important in the expression of disease resistance.

Keywords: acibenzolar-S-methyl; Diplocarpon rosae; induced resistance; intercellular fluid; pathogenesis-related proteins; Rosa hybrida
Subjects: acibenzolar-S-methyl, protection against blackspot disease; blackspot disease, rose, pathogenesis-related proteins; Diplocarpon rosae; pathogenesis-related proteins, blackspot disease; Rosa hybrida

Published: June 1, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Suo, Y., & Leung, D.W.M. (2002). BTH-Induced Accumulation of Extracellular Proteins and Blackspot Disease in Rose. Biologia plantarum, 45(2), 273-279. doi: 10.1023/A:1015161110058
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Benhamou, N., Belanger, R.R.: Benzothiadiazole-mediated induced resistance to Fusarium axysporum f. sp. radicislycopersici in tomato.-Plant Physiol. 118: 1203-1212, 1998. Go to original source...
    2. Bolton, A.T., Svejda, F.J.: A new race of Dilocarpon rosae capable of causing severe black spot on Rosa rugosa hybrids.-Can. Plant Dis. Surv. 59: 38-40, 1979.
    3. Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    4. Burketová, L., ©indelářová, M., ©indelář, L.: Benzothiadiazole as an inducer of β-1,3-glucanase and chitinase isozymes in sugar beet.-Biol. Plant. 42: 279-287, 1999. Go to original source...
    5. Colson-Hanks, E.S., Deverall, B.J.: Effect of 2,6-dichloroisonicotinic acid, its formulation materials and benzothiadiazole on systemic resistance to alternaria leaf spot in cotton.-Plant Pathol. 49: 171-178, 2000. Go to original source...
    6. Dann, E., Diers, B., Byrum, J., Hammerschmidt, R.: Effect of treating soybean with 2,6-dichloroisonicotinic acid (INA) and benzothiadiazole (BTH) on seed yields and the level of disease caused by Sclerotinia sclerotiorum in field and greenhouse studies.-Eur. J. Plant Pathol. 104: 271-278, 1998. Go to original source...
    7. Davies, D.R.: Rapid propagation of roses in vitro.-Scientia Hort. 13: 385-389, 1980. Go to original source...
    8. De Wit, P.J.G.M., Spikman, G.: Evidence for the occurrence of race-and cultivar-specific elicitors of necrosis in intercellular fluids of compatible interactions of Cladosporium fulvum and tomato.-Physiol. Plant Pathol. 21: 1-11, 1982. Go to original source...
    9. Friedrich, L., Lawton, K., Ruess, W., Masner, P., Specker, N., Rella, M.G., Meier, B., Dincher, S., Staub, T., Uknes, S., Métraux, J.-P., Kessmann, H., Ryals, J.: A benzothiadiazole derivative induces systemic acquired resistance in tobacco.-Plant J. 10: 61-70, 1996. Go to original source...
    10. Godard, J.-F., Ziadi, S., Monot, C., Corre, D.L., Silue, D.: Benzothiadiazole (BTH) induces resistance in cauliflower (Brassica oleracea var. botrytis) to downy mildew of crucifers caused by Peronospora parasitica.-Crop Protect. 18: 397-405, 1999. Go to original source...
    11. Görlach, J., Volrath, S., Knauf-Beiter, G., Hengy, G., Beckhove, U., Kogel, K.-H., Oostendorp, M., Staub, T., Ward, E., Kessmann, H.: Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.-Plant Cell 8: 629-643, 1996. Go to original source...
    12. Hochstrasser, D.F., Patchornik, A., Merril, C.R.: Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining.-Anal. Biochem. 173: 412-423, 1988a. Go to original source...
    13. Hochstrasser, D.F., Harrington, M.G., Hochstrasser, A.-C., Mark, J.M., Merril, C.R.: Methods for increasing the resolution of two-dimensional protein electrophoresis.-Anal. Biochem. 173: 424-435, 1988b. Go to original source...
    14. Inbar, M., Doostdar, H., Sonoda, R.M., Leibee, G.L., Mayer, R.T.: Elicitors of plant defensive systems reduce insect densities and disease incidence.-J. chem. Ecol. 24: 135-149, 1998. Go to original source...
    15. Jamet, E., Fritig, B.: Purification and characterization of 8 of the pathogenesis-related proteins in tobacco leaves reacting hypersensitively to tobacco mosaic virus.-Plant mol. Biol. 6: 69-80, 1986. Go to original source...
    16. Jeknic, Z., Chen, T.H.H.: Changes in protein profiles of poplar tissues during the induction of bud dormancy by short-day photoperiod.-Plant Cell Physiol. 40: 25-35, 1999. Go to original source...
    17. Kauffmann, S., Legrand, M., Fritig, B.: Isolation and characterization of six pathogenesis-related (PR) proteins of Samsun NN tobacco.-Plant mol. Biol. 14: 381-390, 1990. Go to original source...
    18. Kauffmann, S., Legrand, M., Geoffroy, P., Fritig, B.: Biological function of pathogenesis-related proteins: four PR proteins of tobacco have 1,3-β-glucanase activity.-EMBO J. 6: 3209-3212, 1987. Go to original source...
    19. Kessmann, H., Staub, T., Hofmann, C., Maetzke, T., Herzog, J.: Induction of systemic acquired disease resistance in plants by chemicals.-Annu. Rev. Phytopathol. 32: 439-459, 1994. Go to original source...
    20. Laemli, U.K.: Cleavage of structural proteins during the assembly of head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    21. Lawton, K.A., Friedrich, L., Hunt, M., Weymann, K., Delaney, T., Kessmann, H., Staub, T., Ryals, J.: Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway.-Plant J. 10: 71-82, 1996. Go to original source...
    22. Legrand, M., Kauffmann, S., Geoffroy, P., Fritig, B.: Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases.-Proc. nat. Acad. Sci. USA 84: 6750-6754, 1987. Go to original source...
    23. Linthorst, H.J.M.: Pathogenesis-related proteins of plants.-Crit. Rev. Plant Sci. 10: 123-150, 1991. Go to original source...
    24. Liu, D., Raghothama, K.G., Hasegawa, P.M., Bressan, R.A.: Osmotin overexpression in potato delays development of disease symptoms.-Proc. nat. Acad. Sci. USA 91: 1888-1892, 1994. Go to original source...
    25. Mauch, F., Mauch-Mani, B., Boller, T.: Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase.-Plant Physiol. 88: 936-942, 1988. Go to original source...
    26. Morris, S.W., Vernooij, B., Titatarn, S., Starrett, M., Thomas, S., Wiltse, C.C., Frederiksen, R.A., Bhandhufalck, A., Hulbert, S.: Induced resistance response in maize.-Mol. Plant Microbe Interact. 11: 643-658, 1998. Go to original source...
    27. Niderman, T., Genetet, I., Bruyère, T., Gees, R., Stintzi, A., Legrand, M.: Pathogenesis-related PR-1 proteins are antifungal.-Plant Physiol. 108: 17-27, 1995. Go to original source...
    28. O'Farrell, P.H.: High resolution two-dimensional electrophoresis of proteins.-J. biol. Chem. 250: 4007-4021, 1975. Go to original source...
    29. Ramagli, L.S., Rodriguez, L.V.: Quantitation of microgram amounts of protein in two-dimensional polyacrylamide gel electrophoresis sample buffer.-Electrophoresis 6: 559-563, 1985. Go to original source...
    30. Schneider, M., Schweizer, P., Meuwly, P., Métraux, J.P.: Systemic acquired resistance in plants.-Int. Rev. Cytol. 168: 303-340, 1996. Go to original source...
    31. Siegrist, J., Glenewinkel, D., Kolle, C., Schmidtke, M.: Chemically induced resistance in green bean against bacterial and fungal pathogens.-J. Plant Dis. Protect. 104: 599-610, 1997.
    32. Stintzi, A., Heitz, T., Prasad, V., Wiedemann-Merdinoglu, S., Kauffmann, S., Geoffroy, P., Legrand, M., Fritig, B.: Plant 'pathogenesis-related' proteins and their role in defense against pathogens.-Biochimie 75: 687-706, 1993. Go to original source...
    33. Suo, Y., Leung, D.W.M.: Induction of resistance to Diplocarpon rosae and Agrobacterium tumefaciens by acibenzolar-S-methyl (BTH) in rose.-J. Plant Dis. Protect.: in press, 2001.
    34. Thompson-Coffe, C., Findlat, T.A.S., Wagner, B.A., Orr, A.R.: Changes in protein complement accompany early organogenesis in the maize ear.-Int. J. Plant Sci. 153: 31-39, 1992. Go to original source...
    35. Towbin, H., Staehelin, T., Gordon, J.: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application.-Proc. nat. Acad. Sci. USA 76: 4350-4353, 1979. Go to original source...
    36. Vigers, A.J., Roberts, W.K., Selitrennikoff, C.P.: A new family of plant antifungal proteins.-Mol. Plant Microbe Interact. 4: 315-323, 1991. Go to original source...
    37. Xue, A.G., Davidson, C.G.: Components of partial resistance to black spot disease (Diplocarpon rosae Wolf) in garden roses.-HortScience 33: 96-99, 1998. Go to original source...
    38. Zhu, Q., Maher, E.A., Masound, S., Dixon, R.A., Lamb, C.J.: Enhanced protection against fungal attack by constitutive co-expression of chitinase and glucanase gene in transgenic tobacco.-Bio/Technology 12: 807-812, 1994. Go to original source...

    Return to the content