biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:281-290, 2002 | DOI: 10.1023/A:1015113226897

Comparison of the Effect of Different Fungal Elicitors on Rubia Tinctorum L. Suspension Culture

K. Bóka1, J. Jakab2, I. Király3
1 Department of Plant Anatomy, Eötvös Loránd University, Budapest, Hungary
2 Agricultural Research Institute of the Hungarian Academy of Science, Martonvásár, Hungary
3 Department of Plant Physiology, Eötvös Loránd University, Budapest, Hungary

The effect of two elicitor types prepared from three different fungi on the alizarin content and the ultrastructure of Rubia tinctorum cells was studied. The de novo alizarin synthesis took place predominantly during the first day of treatment then it was followed by a constant release of alizarin into the medium. The alkalinization of the medium was similar in every treatment. The number of the living cells did not changed during 24-h elicitor treatments, but it decreased significantly after 96 h. The appearance of vacuolar bodies, and the change of the plasmalemmasome structure from vesiculo-reticular to reticulo-lamellar were the most typical morphological syndromes due to elicitation. Phytium elicitor proved to be the least effective, and Botrytis elicitor seemed to be the most effective.

Keywords: alizarin; defence response; fluorescence; madder; ultrastructure
Subjects: alizarin, effect of fungal elicitors; Botrytis cinerea; fluorescence characteristics of suspension cultures, effect of fungal elicitors; fungal elicitors, madder suspension culture; in vitro culture, effect of fungal elicitors; madder, suspension culture, fungal elicitors; Pythium debaryanum; Rubia tinctorum; Verticillium dahliae

Published: June 1, 2002  Show citation

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Bóka, K., Jakab, J., & Király, I. (2002). Comparison of the Effect of Different Fungal Elicitors on Rubia Tinctorum L. Suspension Culture. Biologia plantarum, 45(2), 281-290. doi: 10.1023/A:1015113226897
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