biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:307-310, 2002 | DOI: 10.1023/A:1015173412784

Invertase in Immobilized Cells of Eschscholtzia Californica

K. Neubert1, J. Stano2, K. Mičieta3, P. Kovács2, H. Tintemann1
1 Institute of Biochemistry, M. Luther University, Halle, Germany
2 Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic
3 Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic

Cell suspension culture of Eschscholtzia californica Cham. were permeabilized by Tween 20 or 80, and immobilized by glutaraldehyde. The highest invertase activity was at pH 4.5 and temperature 50 °C. The hydrolysis of the substrate was linear for 5 h reaching 60 % conversion. The cells had high invertase activity and a good stability, and in long-term storage they showed convenient physico-mechanical properties.

Keywords: Californian poppy; cell permeabilization; glutaraldehyde
Subjects: Californian poppy; Eschscholtzia californica; glutaraldehyde, immobilized cells; immobilization, cell; in vitro culture, cell immobilization; invertase, glutaraldehyde, immobilized cells; permeabilization, cell

Published: June 1, 2002  Show citation

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Neubert, K., Stano, J., Mičieta, K., Kovács, P., & Tintemann, H. (2002). Invertase in Immobilized Cells of Eschscholtzia Californica. Biologia plantarum, 45(2), 307-310. doi: 10.1023/A:1015173412784
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