biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:311-313, 2002 | DOI: 10.1023/A:1015125529623

Optimum Assay Conditions of the Activity of Phytochelatin Synthase from Tobacco Cells

R. Nakazawa1, H. Kato1, Y. Kameda1, H. Takenaga1
1 Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan

We determined the characteristics of phytochelatin synthase from tobacco (Nicotiana tabacum cv. Bright Yellow-2) cells, especially the conditions for the enzyme stability. From the results, we proposed the optimum assay conditions of the enzyme activity.

Keywords: metal stress; Nicotiana tabacum
Subjects: heavy metals; in vitro culture, phytochelatin synthase assay; Nicotiana tabacum; phytochelatin synthase, assay conditions; tobacco, phytochelatin synthase

Published: June 1, 2002  Show citation

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Nakazawa, R., Kato, H., Kameda, Y., & Takenaga, H. (2002). Optimum Assay Conditions of the Activity of Phytochelatin Synthase from Tobacco Cells. Biologia plantarum, 45(2), 311-313. doi: 10.1023/A:1015125529623
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