Biologia plantarum 45:315-316, 2002 | DOI: 10.1023/A:1015177513693
Optimum Conditions for the Storage of Potato Virus YNTN Strain
- 1 Institute of Experimental Botany, Academy of Sciences of Czech Republic, Praha 6, Czech Republic
The effect of storage conditions on the serological activity of two isolates of potato virus YNTN strain (PVYNTN) was studied by ELISA. Purified virus, intact and homogenized infected leaves stored freeze-dried and frozen at various temperatures were tested. Purified virus was the most stable at +4 °C and non-purified virus was best preserved as a freeze-dried leaf homogenate at -20 °C. Their serological activity did not change after three months of storage.
Keywords: ELISA; Solanum tuberosum
Subjects: potato virus YNTN strain, storage conditions; Solanum tuberosum
Published: June 1, 2002 Show citation
References
- Avrameas, S.: Coupling of enzymes of proteins with glutaraldehyde. Use of the conjugate for the detection of antigens antibodies.-Immunochemistry 6: 32-52, 1969.
Go to original source... - Beczner, L., Horwath, J., Romhanyi, I., Foerster, H.: Studies on the etiology of tuber necrotic ringspot disease in potato.-Potato Res. 27: 339-352, 1984.
Go to original source... - Čeřovská, N., Filigarová, M., ©ubr, Z.: Optimalization of purification procedure for potato virus Y strain NN.-Acta virol. 41: 47-49, 1997.
- Clark, M.F., Adams, A.N.: Characteristics of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses.-J. gen. Virol. 34: 475-483, 1977.
Go to original source... - Le Romancer, M., Kerlan, C.: La maladie des necroses annulaires superficielles des tubercules: une affection de la pomme de terre due au virus Y.-Agronomie 11: 889-900, 1991.
Go to original source... - Le Romancer, M.: La maladie des necroses annulaires superficielles des tubercules de la pomme de terre. Identification et caractérisation de 1, agent pathogène: le variant YNTN du virus Y de la pomme de terre.-PhD Thesis. University of Rennes, Rennes 1993.
- Le Romancer, M., Kerlan, C., Nedellec, M.: Biological characterization of various geographical isolates of potato virus Y inducing superficial necrosis on potato tubers.-Plant Pathol. 43: 138-144, 1994.
Go to original source... - Steinbuch, M., Audran, R.: The isolation of IgG from mammalian sera with the aid of caprylic acid.-Arch. Biochem. Biophys. 134: 274-284, 1969.
Go to original source... - Weidemann, H.I.: Ringsymptome an Kartoffelknollen Kartoffelvirus Y als vermutliche Ursache.-Kartoffelbau 36: 356-357, 1985.
Go to original source...



