biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:317-319, 2002 | DOI: 10.1023/A:1015129730531

A Rapid and Simple Method for in vitro Plant Regeneration from Split Embryo Axes of Six Cultivars of Cotton

S. Hazra1, A.V. Kulkarni1, A.K. Banerjee1, A.B. Dhage1, D.C. Agrawal1, K.V. Krishnamurthy1, S.M. Nalawade1
1 Plant Tissue Culture Division, National Chemical Laboratory, Pune, India

Plant regeneration was achieved from the seed derived decotyledonated split embryo axes of six Indian cultivars of cotton (Gossypium hirsutum L.). Explants were cultured on Murashige and Skoog's basal medium supplemented with 2 % sucrose and 0.65 % agar. Incorporation of 0.25 % charcoal in the medium and incubation of the cultures at 30 ± 2 °C had synergistic effect on the frequency of shoot and root formation. The method employed is genotype independent, simple and rapid.

Keywords: Gossypium hirsutum; micropropagation; tissue culture; activated charcoal
Subjects: cotton, cultivars, regeneration from split embryo axes; cultivar differences; Gossypium hirsutum; in vitro culture, plant regeneration, organogenesis

Published: June 1, 2002  Show citation

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Hazra, S., Kulkarni, A.V., Banerjee, A.K., Dhage, A.B., Agrawal, D.C., Krishnamurthy, K.V., & Nalawade, S.M. (2002). A Rapid and Simple Method for in vitro Plant Regeneration from Split Embryo Axes of Six Cultivars of Cotton. Biologia plantarum, 45(2), 317-319. doi: 10.1023/A:1015129730531
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