biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:327-336, 2002 | DOI: 10.1023/A:1016201230112

Identification of Flax and Linseed Cultivars by Isozyme Markers

K. Krulíčková1, Z. Poąvec2, M. Griga2
1 Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Brno, Czech Republic
2 Department of Plant Biotechnology, AGRITEC Ltd., ©umperk, Czech Republic

A set of 28 fibre flax and linseed cultivars differing in plant morphology and technological parameters were analysed by isozyme markers in five ontogenetic phases. Relatively high isozyme polymorphism was observed using polyacrylamide gel electrophoresis. Altogether 18 isozyme systems produced 145 different bands; 66 of them (45.52 %) have been found to be polymorphic. The highest level of polymorphism was found in acid phosphatase and esterase, polymorphism was detected in aconitase, diaphorase, glutamate dehydrogenase, peroxidase and superoxide dismutase as well. The highest number of unique isozymic spectra (cultivar × enzyme × ontogenetic phase) was detected in the phase of shoot with removed cotyledons. Electrophoretic analysis of all polymorphic isozymes enabled to distinguish 20 cultivars (71 %) in the screened cultivar set.

Keywords: electrophoresis; Linum usitatissimum L.; native-PAGE; protein polymorphism
Subjects: aconitase, polymorphism; cultivar differences; cultivars, identification by isozyme markers; diaphorase, polymorphism; esterases, polymorphism; flax, isozyme markers; glutamate dehydrogenase polymorphism; linseed, isozyme markers; Linum usitatissimum; markers; native-PAGE; peroxidase; phosphatases; polymorphism, proteins; proteins, polymorphism; superoxide dismutase, polymorphism

Published: September 1, 2002  Show citation

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Krulíčková, K., Poąvec, Z., & Griga, M. (2002). Identification of Flax and Linseed Cultivars by Isozyme Markers. Biologia plantarum, 45(3), 327-336. doi: 10.1023/A:1016201230112
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