biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 45:389-394, 2002 | DOI: 10.1023/A:1016217700979

Effects of Prolonged UV-B Enhanced Fluorescent Radiation on Some Marine Microalgae

C.P.M. Vimalabai1, G. Kulandaivelu1
1 School of Biological Sciences, Madurai Kamaraj University, Madurai, India

The eukaryotic unicellular microalgae Chlorella salina, Dicrateria inornata, and Isochrysis galbana were grown under control (fluorescent 20 W m-2) and UV-B enhanced (UV-BE, 0.5 W m-2) fluorescent radiation. The growth rate showed marginal increase under UV-BE. Decrease in protein content was observed in Dicrateria cells but in Chlorella an initial increase up to 4 d and in Isochrysis an increase at days 4 and 5 was noted. The chlorophyll a content showed marked increase in Chlorella and Isochrysis but in Dicrateria a decline was found. UV-BE reduced the photosynthetic activity in all three species, but the reduction was larger in Chlorella and Dicrateria. Fluorescence excitation spectra for F682 in Chlorella cells grown for 5 d under UV-BE showed reduction in all peaks. In contrast to this, in Dicrateria and Isochrysis cells, the 530 and 590 nm excitation peaks increased with an appreciable decrease in the 466 nm peak. SDS-PAGE analysis revealed significant decrease in the contents of 47, 33, and 23 kDa polypeptides in Chlorella cells. In Dicrateria cells, significant loss in the content of 55, 38, and 18 kDa polypeptides was observed. The content of low molecular mass polypeptides (15 kDa) remained unaffected. Isochrysis cells were more stable in preserving the content of thylakoid polypeptides.

Keywords: chlorophyll; fluorescence spectra; photosynthetic electron transport; phytoplankton; thylakoid proteins
Subjects: Chlorella salina; chlorophyll, ultraviolet-B; 14CO2 fixation, ultraviolet-B enhanced radiation; Dicrateria inornata; fluorescence spectra, chlorophyll, ultraviolet-B enhanced radiation; gas exchange, 14CO2 fixation; Isochrysis galbana; photosynthesis, 14CO2 fixation; photosynthetic electron transport, ultraviolet-B enhanced radiation; phytoplankton, ultraviolet-B enhanced radiation; polypeptides, ultraviolet-B enhanced radiation; thylakoid proteins, UV-B enhanced radiation; ultraviolet-B radiation; UV-B enhanced radiation, marine microalgae

Published: September 1, 2002  Show citation

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Vimalabai, C.P.M., & Kulandaivelu, G. (2002). Effects of Prolonged UV-B Enhanced Fluorescent Radiation on Some Marine Microalgae. Biologia plantarum, 45(3), 389-394. doi: 10.1023/A:1016217700979
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