biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:463-467, 2002 | DOI: 10.1023/A:1016294323269

Isolation and Partial Characterization of Aluminium-Induced Cytoplasmic Polypeptides from Barley Root

L. Tamás1, I. Mistrík1, J. Huttová1
1 Institute of Botany, Slovak Academy of Science, Bratislava, Slovakia

Using preparative isoelectric focusing, fast performance liquid chromatography, and polyacrylamide gel electrophoresis accumulation of several Al-induced cytoplasmic proteins was described in barley roots. Two of them, 27 and 28 kDa polypeptides were isolated by continuous-elution electrophoresis system in sufficient quantities for their further characterisation.

Keywords: IEF; Hordeum vulgare; FPLC; SDS-PAGE
Subjects: aluminium, cytoplasmic polypeptides; aluminium, polypeptide patterns, root; barley, cytoplasmic polypeptides; fast performance liquid chromatography, FPLC; Hordeum vulgare; isoelectric focusing, polypeptides; root, cytoplasmic polypeptides

Published: September 1, 2002  Show citation

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Tamás, L., Mistrík, I., & Huttová, J. (2002). Isolation and Partial Characterization of Aluminium-Induced Cytoplasmic Polypeptides from Barley Root. Biologia plantarum, 45(3), 463-467. doi: 10.1023/A:1016294323269
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